不同日龄惠阳胡须鸡肌肉中IGF1、MyoD基因表达研究

Study on the Expression of IGF1 and MyoD Genes in Huiyang Bearded Chicken at Different Age

已有研究表明,IGF1(胰岛素样生长因子1)、MYOD(肌分化因子)基因在肌细胞的生长、增殖、分化上具有重要的调控作用.本研究以在惠阳胡须鸡为研究对象,采集不同日龄(86、155、213、300、394、454)的惠阳胡须鸡的胸肌和腿肌,然后通过提取RNA、反转录、聚合酶链式反应(PCR)、荧光定量PCR分析了这两个基因在不同日龄惠阳胡须鸡的胸肌和腿肌中的表达情况.半定量结果发现,MYOD基因只在86日龄的腿肌中表达,而IGF1基因在不同日龄的胡须鸡胸腿肌中均有表达,而荧光定量PCR的结果显示IGF1基因在胸腿肌的相对表达量存在差异,但没有达到显著性.这些结果为深入了解IGF1基因在鸡肌纤维生长发育过程的作用提供了新的证据.

It was reported that IGF1(insulin like growth factor1)and MYOD(muscle differentiation factor)genes play an important role in the growth,proliferation and differentiation of muscle cells.In the study samples from breast and leg muscle of Huiyang beared chickens at different day age were collected,followed by RNA extraction,reverse transcription.Polymerase chain reaction(PCR)and fluorescence quantitative PCR were used foranalyzing the expression of the two genesin breast and leg muscleof Huiyang beared chickens at different days in age.The results showed that expression of the MyoD gene is detected in breast and leg muscle of Huiyang beared chickens at86days old,but IGF1gene in breast and leg muscle of Huiyang beared chickens at different days in age.These results provide new evidences for the further understanding of the expression of genes related to growth and development of muscle fiber in chickens.