Bright Yellow 2烤烟热激蛋白90生物信息学分析

Bioinformatics analysis for heat shock protein 90 in flue cured tobacco variety Bright Yellow 2

【目的】分析Bright Yellow 2烤烟热激蛋白90(HSP90)的生物学信息,为揭示烘烤过程中烟叶的烘烤特性提供理论依据。【方法】从NCBI中获取Bright Yellow 2烤烟HSP90蛋白序列Nt HSP-1、Nt HSP-2和Nt HSP-3(Gen Bank登录号分别为BAL42333、BAL42332和BAM24708),利用生物信息学软件对各序列进行理化特性、跨膜区、信号肽、亚细胞定位、二级结构和三级结构预测分析。【结果】Bright Yellow 2烤烟Nt HSP-1、Nt HSP-2和Nt HSP-3的氨基酸数量分别为812、811和699个,均以酸性氨基酸含量较多,其中谷氨酸(Glu)含量最高,理论等电点均小于5.00,二级结构均以α-螺旋(H)和无规则卷曲(C)为主。Nt HSP-1和Nt HSP-2的亲水性较高,具有较高的稳定性,属于分泌蛋白,具有锚定结构,定位于内质网,其三级结构呈现"r"形,N端内部具有Mg2+结合位点、HATPase_c(ATP酶)和top6b(DNA拓扑异构酶VI)功能域;Nt HSP-3为非分泌型蛋白,定位于叶绿体,三级结构呈现"i"形,N端含有Mg2+结合位点和HATPase_c功能域。【结论】Nt HSP-1和Nt HSP-2可能与烟叶耐烤性有关,而Nt HSP-3可能与烟叶易烤性有关。

【Objective】Bioinformatic analysis for heat shock protein90(HSP90)of tobacco(Bright Yellow2)was conducted to provide theoretical basis for revealing characteristics of tobacco leaf curing.【Method】The sequences of NtHSP-1,NtHSP-2and NtHSP-3(respective GenBank accession number:BAL42333,BAL42332and BAM24708),Bright Yellow2HSP90protein members,were collected from NCBI.The physical and chemical properties,transmembrane region,signal peptide,subcellular localization,secondary structure and tertiary structure prediction were analyzed by using bioinformatics software.【Result】Amino acid numbers of NtHSP-1,NtHSP-2and NtHSP-3were812,811and699respectively.The content of acidic amino acid was the majority,with glutamic acid(Glu)accounting for the highest content.Theoretical isoelectric points of them were all less than5.00,and the secondary structures were mainlyα-helix(H)and irregular curl(C).NtHSP-1and NtHSP-2presented high hydrophilicity and stability.They belonged to secretory protein with anchor structure,and located in endoplasmic reticulum.Their tertiary structures were in“r”shape,having a Mg2+binding site,HATPase_c(ATPase)and top6b(DNA topoisomerase VI)functional domain inside N-end.NtHSP-3was a non-secreted protein which located in chloroplast.Its tertiary structure was in“i”shape,containing a Mg2+binding site and HATPase_c functional domain at N-end.【Conclusion】NtHSP-1and NtHSP-2may be related to endurable curing potential of tobacco leaves during curing.NtHSP-3may be related to easy curing potential of tobacco leaves during curing process.