浙江近海海域浮游病毒和异养细菌的时空分布

The spatial and temporal distribution of virioplankton and heterotrophic bacteria in the coastal waters of Zhejiang

为了解浙江近海海域浮游病毒和异养细菌的时空生态分布,于2014年11月(秋)、2015年1月(冬)、2015年5月(春)和2015年7月(夏)连续4个季节采集了浙江近海海域表层海水样品,采用流式细胞仪技术对样品浮游病毒和异养细菌丰度进行了检测,对其时空分布特征及与环境因子的相关性做了分析。从水平分布来看,在4个季节中浮游病毒、异养细菌丰度均为宁波、沈家门、岱山等沿岸海域站位的丰度低,远陆海域东极和枸杞站位的丰度高。从季节变化来看,浮游病毒、异养细菌丰度的季节分布特征同为夏>春>秋>冬,相关性分析结果表明,春、夏、秋、冬4个季节,浮游病毒丰度与异养细菌丰度均为显著正相关。浮游病毒丰度在春、秋、冬季节均与病毒/细菌比值(VBR)显著正相关;夏、秋季节均与盐度显著正相关;春、夏季节均与总磷显著负相关;春季分别于与溶解氧、pH、化学耗氧量(COD)显著正相关。异养细菌在春、秋、冬季节均与VBR显著正相关;春、夏季节与溶解氧显著正相关,冬季与溶解氧显著负相关;春、夏季节与总磷显著负相关;秋、冬季节均与温度、盐度显著正相关;春、冬季节均与COD显著正相关。

In order to investigate the spatial and temporal distribution of virioplankton and heterotrophic bacteria in the coastal waters of Zhejiang,the representative samples of sea water were collected in the coastal waters of Zhejiang for4consecutive seasons in November2014(Autumn),January2015(winter),May2015(spring)and July2015(summer).The distribution of virioplankton and heterotrophic bacteria was studied by flow cytometry,and the correlations between the abundances of virioplankton,heterotrophic bacteria and environmental factors were also analyzed.From the horizontal distribution,in the four seasons low abundance of virioplankton and heterotrophic bacteria was in the stations of Ningbo,Shenjiamen,Daishan immediate offshore area;high-abundance was in the stations of Dongji and Gouqi offshore area.From the seasonal distribution,the abundance of the virioplankton and heterotrophic bacteria was the same as that of summer>spring>autumn>winter.The abundance of virioplankton and heterotrophic bacteria was significantly and positively correlated in the4seasons.The abundance of virioplankton was significantly and positively correlated with VBR in spring,autumn and winter.The abundance of virioplankton was significantly and positively correlated with salinity in summer and autumn.The abundance of virioplankton was significantly and negatively correlated with TP in spring and summer.The abundance of virioplankton was significantly and positively correlated with dissolved oxygen,pH,COD in spring.The heterotrophic bacteria were significantly and positively correlated with VBR in spring,autumn and winter.The heterotrophic bacteria were significantly and positively correlated with dissolved oxygen in spring and summer but were significantly and negatively correlated with dissolved oxygen in winter.The heterotrophic bacteria were significantly and negatively correlated with TP in spring and summer.The heterotrophic bacteria were significantly and positively correlated with temperature and salinity in autumn and winter.The heterotrophic bacteria were significantly and positively correlated with COD in spring and winter.