摘要
以地热河谷地区的薏苡仁为研究对象,提取薏苡仁油并进行抗肿瘤活性测定,目的是为提高薏苡仁油的得率,为工业化提取薏苡仁油提供参考。以提取时间(30-50min)、提取温度(40-60℃)、料液比(1︰2.5-1︰4.5m/v)和超声功率(100-140W)为因素,采用L9(34)正交试验优化了最佳工艺,并进一步进行薏苡仁油对肿瘤细胞的抑制活性研究。结果表明,上述4个因素对高良乡薏苡仁油提取率均有一定的影响,提取时间、提取温度、料液比和超声功率对薏苡仁油提取率的贡献率分别是74.47%、16.53%、5.11%和2.83%,并通过直观分析和方差分析,得知薏苡仁油的最佳提取工艺为以无水乙醇为溶剂,料液比1︰2.5m/v、提取温度60℃、提取时间50min、超声功率100W,在此提取条件下薏苡仁油的提取率为7.68±0.12%,采用该提取工艺得到的薏苡仁油对结肠癌细胞(SW480)、人乳腺癌细胞(MCF7)、人肝癌细胞(SMMC7721)、人肺癌细胞(A549)、人早幼粒白血病细胞(HL-60)5种肿瘤细胞的抑制率分别为95.52%、85.02%、91.83%、92.54%、72.89%。该研究结果为将薏苡仁油开发成抗肿瘤保健植物油奠定理论基础。
In order to improve extraction yield of coix-seed oil and provide a reference for industrialization,coix from low-hot valley was targeted as research object,coix-seed oil was extracted and its antineoplasmic activity were studied in this paper.Methods for improving extraction yield of coix-seed oil were optimized through L9(34)orthogonal experiments with the factors including extraction time(30-50min),extraction temperature(40-60℃),ratio of material to liquid(1:2.5-1:4.5m/v),ultrasonic power(100-140W).Furthermore,inhibiting effect of coix-seed oil on some cancer cells was tested.The results showed that yield of oil from seed of coix was significantly affected by four factors,contribution rate of extraction time,extraction temperature,ratio of material to liquid and ultrasonic power,which were74.47%,16.53%,5.11%,2.83%,respectively.The optimized extraction method was confirmed based on visual and variance analysis that absolute ethyl alcohol was considered as optimal extraction solvent,with optimal extraction time,extraction temperature,ratio of material to liquid and ul trasonic power of50min,60°C,1:2.5m/v,100W,respectively.Under these conditions,extraction yield of oil from seed of coix was7.68±0.12%?A n d collected oil from coix seed could prevent growth of colon cancer cell(SW480),human breast cancer cell(MCF7),hepatoma carcinoma cell(SMMC7721),human lung cancer cell(A549),and leukemia cell(HL-60)at inhibiting rate of95.52%,85.02%,91.83%,92.54%,72.89%,respectively.The results of the study may lay a theoretical foundation for developing coix-seed oil as health-care vegetable oil with antineoplastic activity.
作者
张静美
施蕊
夏菁
李彪
赵一鹤
ZHANG Jing-mei;SHI Rui;XIA Jing;LI Biao;ZHAO Yi-he(Yunnan Forestry Technological College, Kunming Yunnan 650224, P.R.China;Key Laboratory of the Ministry of Education for Agro-Biodiversity and Pest Management, Yunnan Agricultural University, Kunming Yunnan 650201, P.R.China;Key Laboratory of Biodiversity Conservation and Use in Southwest China, State Forestry Administration, Southwest Forestry University, Kunming Yunnan 650224, P.R.China;Yunnan Academy of Forestry, Kunming Yunnan 650201, P.R.China)
出处
《西部林业科学》
CAS
2017年第5期113-118,共6页
Journal of West China Forestry Science
基金
云南省森林植物培育与开发利用实验室开放基金(2016)
云南省社会事业发展专项(2010CA010)资金资助
关键词
薏苡仁油
正交试验
癌细胞
抑制作用
coix-seed oil
orthogonal experiment
cancer cell
inhibiting effect