抗核抗体对孕妇梅毒抗体检测干扰

Observation on the Interference of Anti-nuclear Antibody on Detection of Syphilis Antibody in Pregnant Women

目的探讨抗核抗体(ANA)及9种抗ENA抗体(Ro-52,SSA,SSB,Jo-1,RNP/Sm,Sm,Scl-70,Sm,Scl-70,PM-Scl,ds DNA)对孕妇梅毒抗体(TP-Ab)检测的干扰及影响.方法经ELISA方法检测138例抗核抗体阳性孕妇(实验组1)、55例抗核抗体阴性孕妇(实验组2)和60例非孕妇健康体检者(健康对照组)血清TP-Ab抗体,合计253例,并分析ANA及其9种抗ENA抗体的TP-Ab检出情况,运用TPPA法进行确认评价;各组间率的比较采用χ~2.结果实验组1中TP-Ab阳性12例占8.70%(12/138),显著高于健康对照组全部阴性(P<0.05);2组间[实验组2中TP-Ab阳性1例占1.82%(1/55)与健康对照组中TP-Ab全部阴性]差异无统计学意义(P>0.05);抗ENV抗体的TP-Ab阳性仅出现在Ro-52和SSA,阳性率分别为14.71%(10/68)和5.40%(2/37),2组间差异无统计学意义(P>0.05);253标本TPPA结果均为阴性.结论 ANA可干扰TP-Ab的检测,造成假阳性结果,尤其是Ro-52引起的发生率较高.

Objectives To investigate the effects of anti-nuclear antibody(ANA)and nine anti-ENA antibodies(Ro-52,SSA,SSB,Jo-1,RNP/Sm,Sm,Scl-70,Sm,Scl-70,PM-Scl,dsDNA)on the detection of syphilis antibody in pregnant women.Methods A total of253cases was recruited,with138anti-nuclear antibody positive pregnant women(experimental group1),55anti-nuclear antibody negative pregnant women(experimental group2)and60healthy non-pregnant women(healthy control group)and serum TP-Ab was detected with ELISA as screening method.The positive incidents of TP-Ab of ANA and its nine anti-ENA antibody were confirmed by TPPA method.Chi-square test was used to compared positive rate.Results There were12cases of TP-Ab positive in the experimental group1,accounting for8.70%(12/138),which was significantly higher than that in the healthy control group(P<0.05),while there was no significant difference(P>0.05)between experimental group2and control group,with TP-Ab rate of1.82%(1/55)and zero re.positive antibody TP-Ab of Anti-ENV antibody were only detected in Ro-52and SSA,which of positive incidence accounted for14.71%(10/68)and5.40%(2/37)respectively,there was significant difference between the two groups(P<0.05).The results in253cases were totally confirmed negative through TPPA method.Conclusions ANA can interfere with the detection of TP-Ab,which caused false positive results,especially the risk of falsepositivity caused by Ro-52is higher.