迷迭香干预三重脑震荡大鼠焦虑行为变化

Effects of Rosemary on Anxiety of Rats with Multiple Concussions

目的用迷迭香干预多重脑震荡(multiple cerebral concussion,MCC)大鼠,观察其抗焦虑治疗作用.方法用单摆打击装置复制大鼠三重脑震荡(3MCC)模型.将损伤大鼠随机分成迷迭香高、中、低3个剂量组、吐温溶剂组、3MCC组和正常对照组(C组).伤后连续给药16 d后,进行旷场实验(OFT),评估其焦虑样行为改变情况.结果 (1)与正常组比较:各实验组在旷场中央区活动时间均减少,在旷场周围区活动时间增加,除高剂量组外,其余各组差异均有统计学意义(P<0.05);各实验组在总穿格数、中央穿格数均减少,且差异有统计学意义(P<0.05);在周边穿格数检测中,除中剂量组外,其余各组均减少,且低剂量组、3MCC组、吐温组差异有统计学意义(P<0.05);(2)与3MCC组比较:各药物干预组在旷场中央区活动时间、总穿格数均明显多于损伤组,在旷场周边区活动时间明显少于损伤组,差异有统计学意义(P<0.05);在旷场中央与周围穿格数中除低剂量组外,均明显多于损伤组,差异有统计学意义(P<0.05).在大鼠直立次数中各实验组差异无统计学意义(P>0.05).结论三重脑震荡大鼠伤后17 d出现了明显的焦虑样行为改变,迷迭香伤后早期干预可明显改善多重脑震荡大鼠的伤后焦虑样行为.

Objective To investigate the possible effects of rosemary on anxiety in rats with multiple concussions.Methods The rat model of three-fold cerebral concussion(3MCC)was performed using a metallic pendulum striker concussive device.Then the rats were randomly divided into six groups:high dose of rosemary intervention group(H),medium dose of rosemary intervention group(M),low doses of rosemary intervention group(L),tween control group(T),Normal control group(N),3MCC injury group(I).The effect of regular rosemary infusion on anxiety was assessed by open field test.Results(1)Compared with N group:rats of the rest of the groups spent less time in the central area and more time in the surrounding area.The number of total grid,and central grid,however,the number of surrounding grid were less in all groups except M group.(2)Compared with I group:Rats in rosemary intervention groups spent more time in central area and less time in surrounding ares.And the number of total grid in rosemary intervention groups were more.The number of central grid and surrounding grid were obviously more than I group.Moreover,rearing time among all groups have no significant changes.Conclusion The rats can develop anxiety on the17th day after three-fold cerebral concussion,and rosemary has a significant anti-anxiety affect on multiple concussions.