酶标分析仪检定结果的不确定度分析评定

A evaluation for uncertainty analysis of the verification result of ELISA analyzer(microplate reader)

目的:分析酶标分析仪检定中影响检定结果的各种因素,评估酶标分析仪检定结果的准确性。方法:通过检定结果的比对和测量标准重复性的计算,分析和评定标准装置的准确性。结果:经过分析被检仪器操作和标准装置的重复性对检定的影响,排除了检定中影响检定结果的各种因素,确保了检定结果的准确性。结论:在检定酶标分析仪过程中,注意被检仪器的设置,按照规程操作可以确保分光光度计的量值准确可靠,并注意检定结果的分析,确定其重复性是否满足要求。

Objective:To analyze various factors that affect the verification result in the verification by using ELISA analyzer and assess the accuracy of verification results of ELISA analyzer.Methods:Through the comparison of verification results and the calculation for the repeatability of measurement standard,the accuracy of standard device was analyzed and verified.Results:Through analyzed the effect of repeatability of operation and standard device,the factors that affected verification results were eliminated.And the accuracy of verification results were guaranteed.Conclusion:In the process of verification for ELISA analyzer,operators should pay attention to the setting of the verification instrument.And the operation regulation can ensure the accuracy and reliability of the measured value of spectrophotometer.Besides,operators also should pay attention to the analysis of verification results and confirm whether the repeatability of the verification results can meet the requirement of practice.