杜仲的核磁共振指纹图谱研究

NMR Fingerprints of Eucommia ulmoides Oliver

核磁共振氢谱(Proton Nuclear Magnetic Resonance,~1H NMR)是中药指纹图谱中一种鉴定和控制植物中药质量的新方法.本文采用CPMG(Carr-Purcell-Meiboom-Gill)脉冲序列采集了杜仲提取物的~1H NMR谱,通过完整还原振幅频率表(Complete Reduction to Amplitude-Frequency Table,CRAFT)分析技术对杜仲指纹图谱进行特征指纹分析,将待目标合物的信号从混合物图谱中剥离出来,实现了不分离样品而分析目标化合物信息的目的,从而对杜仲的特征化合物——松脂醇二葡萄糖苷(Pinoresinol Glucoside,PDG)进行了定性和定量分析.结果显示贵阳药用植物园所得杜仲的PDG含量为0.275 6%,相对标准偏差(Relative Standard Deviation,RSD)为1.69%,与高效液相色谱(High Performance Liquid Chromatography,HPLC)定量分析结果(含量为0.269 6%,RSD为0.65%)基本一致.另外,通过NMR检测与多变量数据建模相结合分析了杜仲提取物的全指纹图谱,结果显示同一采收期不同产地的杜仲药材有显著差异,这表明该方法可用于鉴定不同产地的药材,具有一定的实用意义.

The1H NMR fingerprint of Eucommia ulmoides acquired with a CPMG pulse sequence was analyzed with the complete reduction to amplitude-frequency table(CRAFT)approach.The signals of target compounds were extracted without chemical separation and purification.Quantitative analysis showed that the average concentration of pinoresinol glucoside(PDG)in Eucommia ulmoides got from Guiyang medical plant garden was0.2756%with a relative standard deviation(RSD)of1.69%.The results were consistent with those obtained by high performance liquid chromatography(average content of0.2696%and RSD of0.65%).NMR fingerprint and multivariate statistical analysis also revealed that there existed significant differences between Hunan and Guizhou Eucommia ulmoides Oliver.