红花蜂花粉多糖提取工艺的研究

Comparative Research on the two Extraction Processes of Polysaccharides from Bee Pollen of Carthamus tinctorius

目的:研究红花蜂花粉多糖的最佳分离提取工艺。方法:以多糖提取率为考察指标,用水提醇沉法和微波提取法分离提取红花蜂花粉多糖。通过单因素试验和L_9(3~4)正交试验,比较两种方法对多糖得率的影响。结果:工艺验证实验结果表明,微波提取法最佳提取工艺为料液比1:18 g/mL、功率500 W、时间7 min,多糖提取率为34.60%;水提醇沉法最佳提取工艺为:料液比1:18 g/mL、时间10 h、温度80℃、提取次数4次,多糖提取率为38.00%。结论:水提醇沉法比微波提取法的多糖得率高,工艺路线稳定可行,可作为红花蜂花粉多糖分离提取的最佳工艺路线。

Objective:To study the best extracting technique of polysaccharides from bee pollen of Carthamus tinctorius.Methods:Taking the yield of polysaccharide as indicator,the polysaccharide of bee pollen of Carthamus tinctorius L was isolated by water extracting-alcohol precipitating method and microwave power method.Then by the use of orthogonal experiment and single factor test,the results of the two methods on the extraction yields of polysaccharides were compared.Results:The results of verification tests showed that the best extraction technology of microwave power method was1:18g/mL for the ratio of material to liquid,500watts for microwave power and7min for extraction time.Under these process conditions,the yield of polysaccharide was34.60%.And the best conditions of water extracting-alcohol precipitating method was1:18g/mL for the ratio of material to liquid,80℃for extracting temperature,10h for time and4times for extraction.The yield of polysaccharide was38.00%.Conclusion:The yield of polysaccharide of water extracting-alcohol precipitating method is better than the microwave power method.It is stable and feasible to use this method as the best extraction process of polysaccharides from bee pollen of Carthamus tinctorius.