沸石曝气生物滤池短程硝化特性及其机制

Partial nitrification performance and mechanism of zeolite biological aerated filter for ammonium wastewater treatment

通过改变进水氨氮浓度和容积负荷,研究沸石曝气生物滤池(ZBAF)的短程硝化特性及其机制,利用高通量测序技术分析ZBAF中生物膜的微生物群落结构.结果表明:稳定运行阶段,亚硝氮产率(NPR)可达0.760kg/(m^3·d),亚硝氮积累率(NAR)高于98%;游离氨(FA)对亚硝酸盐氧化菌(NOB)的抑制是实现短程硝化的主要原因,沸石对氨氮的吸附作用有利于维持合适的FA浓度,从而选择性抑制NOB的活性;亚硝氮的产生遵循零级动力学模型;ZBAF内实现了AOB的富集以及NOB的抑制,其中AOB(Nitrosomoadaceae)的相对丰度高于61%,未检测出NOB.

The ammonia concentration and nitrogen loading rate of influent were varied to investigate the performance and mechanism of partial nitrification in the zeolite biological aerated filter(ZBAF)reactor.The microbial community structure of biofilm on the surface of zeolite was studied by high-throughput sequencing analysis technique.In the steady state,the nitrite production rate(NPR)was0.760kg/(m3·d)and the nitrite accumulation rate was higher than98.0%.The inhibition of free ammonia(FA)to the nitrite oxidizing bacteria(NOB)was proved to be the main reason of partial nitrification.Due to the adsorption of zeolite to ammonium,FA concentration could be remained at an appropriate range for inhibition of nitrite oxidizing bacteria(NOB).The production of nitrite in ZBAF followed the zero-order kinetics model.The enrichment of AOB and inhibition of NOB were realized in ZBAF,and the relative abundance of AOB(Nitrosomoadaceae)was more than61%while no NOB was detected.