添加芽孢杆菌污泥反硝化特性及菌群结构分析

Denitrification characteristics of Bacillus subtilis sludge and analysis of microbial community structure

以普通活性污泥为对照,研究投加芽孢杆菌菌剂的活性污泥(以下称芽孢杆菌活性污泥)的反硝化性能,以及芽孢杆菌对微生物菌群结构的影响.结果表明,在一定范围内两污泥反硝化速率与碳氮比呈正相关,实验组污泥在碳氮比为10时最大比反硝化速率可达27.144mg TN/(g MLSS·h),约为对照组的2.7倍.利用Miseq高通量测序技术对比两组污泥菌群结构,发现在细菌门、纲、目、科、属层面实验组菌群多样性均优于对照组,在细菌属水平上实验组污泥主要包括芽殖杆菌属(Gemmobacter)、短单胞杆菌属(Brachymonas)、热单胞菌属(Thermomonas)、Defluviimonas、长绳菌属(Longilinea)、Ornatilinea、Aridibacter、微小杆菌属(Exiguobacterium)等优势菌属,在碳源充足的条件下这些细菌协同作用,使芽孢杆菌活性污泥具有高效反硝化特性.

Compared with the conventional activated sludge,the experiment was carried out to study the denitrification performance of activated sludge of Bacillus subtilis(hereinafter called Bacillus activated sludge)and the effect of Bacillus on microbial community structure.The results showed that within a certain range,the denitrification rate of two sludge was positively correlated with carbon-nitrogen ratio,the maximum denitrification rate of the experimental group could reach27.144mg TN/(gMLSS·h)with the carbon-nitrogen ratio of10,about2.7times of the control group.The bacterial community structure of the two groups was compared by Miseq high-throughput sequencing,and the results showed that the diversity of bacterial community in the experimental group of bacterial phylum,class,order,family and genus was better than that of the control group.The sludge in the experimental group mainly included Gemmobacter,Brachymonas,Thermomonas,Defluviimonas,Longilinea,Ornatilinea,Aridibacter,Exiguobacterium and so on.These bacteria cooperated to make the activated sludge with high efficient denitrification characteristics with sufficient carbon source.