硫化氢对心肌缺血/再灌注损伤炎症因子的影响

Influence of hydrogen sulfide on inflammatory factors after myocardial iscehemia-reperfusion injury

目的观察硫化氢(H_2S)对心肌缺血/再灌注损伤的影响。方法 24只SD大鼠随机分为A组、B组和C组,各8只,构建Langendorff离体大鼠心脏灌注模型,分别予以KH液、KH液+NaHS(由HC1和Na2S临用前配制,浓度为1μM)和STH液进行灌注。在T0(手术开始前30min)、T1(心脏停搏后15min)、T2(心脏停搏后30 min)、T3(心脏复跳后15 min)、T4(心脏复跳后30 min)时,采用ELISA法测定灌流液中低氧诱导因子-1α(HIF-1α)及白介素-6(IL-6)、白介素-10(IL-10)、肿瘤坏死因子-α(TNF-α)表达情况。H9C2心肌细胞分为组1、组2和组3,分别予KH液、KH液+NaHS、STH液保存6h后恢复室温,ELISA法检测细胞上清中HIF-1α、IL-6、IL-10和TNF-α表达。结果与术前比较,停搏后各组心肌HIF-1α表达均有所下调,差异有统计学意义(P均<0.05)。与A组和C组比较,B组HIF-1α的表达在T2、T3和T4各时间点均升高,差异有统计学意义(P均<0.05)。与术前比较,各组大鼠心脏停搏后IL-6、IL-10以及TNF-α的表达均增加,差异有统计学意义(P均<0.05)。B组IL-6、IL-10以及TNF-α的表达在T2、T3和T4各时间点均低于A组和C组,差异有统计学意义(P均<0.05)。与组1、组3相比,组2细胞上清的HIF-1α表达明显升高,IL-6、IL-10、TNF-α的表达明显降低,差异有统计学意义(P均<0.05)。结论硫化氢降低心肌缺血/再灌注损伤的炎症反应水平,增加HIF-1α的表达,发挥心肌保护作用。

Objective To observe the influence of hydrogen sulfide(H2S)on myocardial ischemiareperfusion injury.Methods SD rats(n=24)were randomly divided into groups A,B and C(each n=8),and model of Langendorff cardiac perfusion in vitro was established.The groups were given,respectively,perfusion of KH solution,KH solution+NaHS(prepared with HCl and Na2S just before using,1μM)and STH solution.At the time points of T0(30min before the operation),T1(15min after asystole),T2(30min after asystole),T3(15min after heart resuscitation)and T4(30min after heart resuscitation),the expressions of hypoxia inducible factor-1α(HIF-1α),interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor-α(TNF-α)in perfusion solution were detected by using enzyme-linked immunosorbent assay(ELISA).H9C2myocardial cells were divided into groups1,2and3,given,respectively,KH solution,KH solution+NaHS and STH solution,stored for6h and then recovered to room temperature.The expressions of HIF-1α,IL-6,IL-10and TNF-αin cell supernatant were detected by using ELISA.Results The expression of HIF-1αdecreased in all groups after asystole compared with before(all P<0.05).The expression of HIF-1αincreased in group B compared with group A and group B at time points of T2,T3and T4(all P<0.05).The expressions of IL-6,IL-10and TNF-αincreased in all groups after asystole compared with before(all P<0.05).The expressions of IL-6,IL-10and TNF-αwere lower in group B than those in group A and group C at time points of T2,T3and T4(all P<0.05).The expression of HIF-1αincreased significantly,and expressions of IL-6,IL-10and TNF-αdecreased significantly in group2compared with group1and group3(all P<0.05).Conclusion H2S can reduce inflammatory reactions,improve expression of HIF-1αand play a role of protecting myocardium in rats with myocardial ischemia-reperfusion injury.