视神经脊髓炎转基因(2D2)鼠的繁殖和鉴定

Reproduction and genotype identification of 2D2 transgenic mouse model of neuromyelitis optica

目的建立视神经脊髓炎转基因(2D2)小鼠的繁育方法,并对其子代鼠进行基因鉴定。方法将雌雄2D2转基因小鼠各1只与C57BL/6野生型小鼠(1∶1)进行交配,由鼠尾血提取基因组DNA,应用PCR技术扩增,电泳后观察2D2转基因传代小鼠的基因表型。结果共繁育产生56只子代小鼠,其中36只子代小鼠基因组DNA扩增出657bp的2D2条带,阳性率为64.3%。结论成功利用杂交方式有效繁殖2D2转基因鼠和应用PCR技术进行基因鉴定,为后续视神经脊髓炎的实验研究奠定了基础。

ObjectiveTo breed and identify the genotype of2D2transgenic mouse model of neuromyelitis optica MethodsThe male and female2D2transgenic mice mated with C57BL/6wild type mice respectively(1∶1).Genomic DNA was isolated from blood cells from the tails and analyzed by PCR ResultsThere were362D2transgenic mice identified by PCR in the generated56mice,and the657bp2D2genome DNA were detected in these2D2transgenic baby mice The positive rate was643%ConclusionsThe appropriate methods of breeding is the effective way to acquire heterozygote baby mice with2D2transgenic mouse It provides the foundation for the future experimental study of neuromyelitis optica