RT-qPCR技术在法医病理学研究中的应用

Application of RT-qPCR in the Study of Forensic Pathology

实时荧光定量逆转录聚合酶链反应(reverse transcription quantitative real-time polymerase chain reaction,RT-qPCR)是一种易操作且高效率检测组织或体液样本中m RNA的方法 ,具有操作容易、敏感性高、特异性强等特点。随着其在医学、生物学等领域的广泛应用,RT-qPCR技术在法医病理学领域的研究也得到一定发展。本文对RT-qPCR技术在法医病理学科研工作中的应用价值及现状进行综述,对国内外研究进展进行了回顾,并总结了实验过程中的检材提取、RT-qPCR实验及数据处理的注意事项,以期为法医学研究及应用提供参考。

Reverse transcription quantitative real-time polymerase chain reaction(RT-qPCR)is a convenient and highly efficient method for the detection of mRNA in tissues or body fluid samples.It has the characteristics of easy operation,high sensitivity and specificity,etc.With a wide application in medicine,biology and other fields,RT-qPCR technique has made some progresses in the research field of forensic pathology.This paper reviews the application value of RT-qPCR in the study of forensic pathology and current situation,as well as the research progress at home and abroad reviews.It also summarizes the notes of samples extraction,RT-qPCR experiments and data processing,which aims to provide reference for the forensic research and its application.