恒古骨伤愈合剂对骨折树鼩血液生理和脏器系数的影响

Effect of Osteoking on blood physiological parameters and organ indexes in female tree shrews with osteoporotic fracture

目的评价恒古骨伤愈合剂对去势树鼩骨质疏松性骨折模型的血液生理指标和脏器系数的影响。方法 30只雌性树鼩分为对照组、模型组和治疗组。治疗组和模型组树鼩切除双侧卵巢6个月证实骨质疏松后再造成胫骨骨折。于骨折次日起,治疗组用恒古骨伤愈合剂灌胃,每两天1次;模型组和对照组用等量生理盐水灌胃。灌胃3个月后,检测各组树鼩血液生理指标和脏器系数。结果在16项血液生理指标中,模型组白细胞数、淋巴细胞绝对值和百分率显著低于对照组(P<0.01),单核细胞百分比和绝对值也降低(P<0.05)。与模型组比较,治疗组单核细胞百分比和绝对值显著升高(P<0.01),淋巴细胞绝对值和百分率以及白细胞数显著升高(P<0.05),且治疗组脾脏系数明显高于模型组(P<0.05)。结论恒古骨伤愈合剂提高骨质疏松性骨折树鼩的免疫力。

ObjectiveTo evaluate the effect of Osteoking on blood physiological parameters and organ indexes in the ovariectomized tree shrew model of osteoporotic fracture(OPF).MethodsA total of304month old female tree shrews(Tupaia belangeri)were randomly divided into control group,model group and treatment group,with10in each group.Bilateral ovaries of the tree shrews in the treatment group and model group were removed and osteoporosis was confirmed6months later.Then tibial fractures were generated.From the next day of fracture,tree shrews in the treatment group were gavaged with Osteoking once every two days,and tree shrews in the model group and control group were gavaged with equal volume of sterile saline.After gavaging for3months,16blood physiological parameters and6organ indexes of the tree shrews in each group were measured.ResultsAmong all of the16blood physiological parameters,the number of white blood cells,the absolute value and percentage of lymphocytes(P<001)and monocytes(P<005)in the model group were significantly lower than those in the control group after3months.Compared with the model group,the absolute value and percentage of monocytes(P<001)and lymphocytes,the number of white blood cells(P<005)and the spleen coefficient were significantly increased(P<005)in the treatment group.ConclusionsOsteoking can improve the immunity of tree shrews with osteoporotic fracture.