移液枪在小鼠内眦取血中的应用

Use of a pipettor for blood collection from the inner canthus of mice

目的介绍移液枪用于小鼠内眦取血的方法。方法 20只C57BL/6J雄性小鼠,分为移液枪组和毛细管组,分别用移液枪和毛细管进行小鼠内眦定量取血3次,记录两组采血完成时间和采血成功率。结果两组采血成功率为100%,但移液枪组采血完成时间快于毛细血管组,且差异有显著性(P<0.05)。结论直接应用移液枪为小鼠内眦取血提供了一种简便易行且相对安全的方法。

ObjectiveTo explore the practical value of a modified method for blood collection from the inner canthus of mice using a pipettor.MethodsA total of20male C56BL/6J mice were divided into two groups,the pipettor group and the capillary group(10mice in each group).Blood samples were taken3times from the inner canthus of the mice with a pipettor or glass capillary,respectively,and the time consumption and success rate of the two groups were recorded and compared.ResultsThe success rates of both the blood collection methods were100%.However,in the pipettor group,the blood collection was completed faster than the capillary group with a significant difference(P<005).ConclusionsIt is a relatively simple and safe method for blood collection from the inner canthus of mice using a pipettor.