红光治疗仪联合胰岛素治疗2型糖尿病足模型大鼠的实验研究

Experimental study of red light therapy instrument combined with insulin in type 2 diabetic foot model rats

目的:探究红光治疗仪联合胰岛素治疗对2型糖尿病足(DF)大鼠伤口的治疗作用与临床应用价值。方法:通过向Wistar大鼠腹腔内注射中等剂量高糖高脂链脲佐菌素,并严格控制室温,用高糖高脂链脲佐菌素(STZ)复制2型糖尿病Wistar大鼠,进行适当足部介入手术来建立2型糖尿病足大鼠模型。大鼠模型被随机分为4组:二甲双胍0.140g/kg组、红光治疗仪联合胰岛素治疗组、模型组、空白对照组(对大鼠灌胃给药30d),进一步观察每组大鼠伤口愈合率、胰岛素、血糖血脂及血管内皮活性因子等实验室指标的变化。结果:红光治疗仪联合胰岛素治疗组大鼠的各项观察指标改善最为明显,差异有统计学意义(P<0.05),明显促进DF大鼠创面伤口愈合,提高愈合率、升高胰岛素敏感性,提高胰岛素敏感指数(ISI)、显著延长凝血四项时间,大鼠血清中一氧化氮(NO)、一氧化氮合酶(NOS)等含量增加,DF模型大鼠胰岛素抵抗、血小板聚集率、血糖、血脂、血液黏度和内皮素(ET)等活性因子明显降低。结论:(1)红光治疗仪治疗可以通过改善血液流变学特性、改善糖脂类代谢、提高胰岛素敏感性、促进血管内皮细胞功能活化等作用,提高DF模型大鼠创面伤口愈合率。(2)红光治疗仪治疗疗效确切,安全性高,临床应用价值高。

Objective:To investigate the therapeutic effect and clinical value of red light therapy combined with insulin therapy on wound in type2diabetic foot(DF)rats.Methods:Wistar rats were injected intraperitoneally with medium dose high fat and high fat streptozotocin and carefully controlled at room temperature.The type2diabetes mellitus Wistar rats were reproved with high glucose and high fat streptozotocin(STZ)Foot involvement in the establishment of type2diabetic foot rat model.The rats were randomly divided into three groups:metformin0.140g/kg group,red light therapy group combined with insulin treatment group,model group,blank control group(30d after intragastric administration of rats),further observation of each group of rat wounds Healing rate,insulin,blood glucose and blood lipid and vascular endothelial activity factors such as laboratory changes.Results:The observation of red light therapy combined with insulin treatment group was the most obvious,the difference was statistically significant(P<0.05),significantly promoted wound healing in DF rats,increased healing rate,increased insulin sensitivity(NO)and nitric oxide synthase(NOS)were increased in the serum of rats.The insulin resistance and platelet aggregation rate of the DF model rats were significantly higher than those of the control group(P<0.05),and the insulin sensitivity index(ISI),Blood glucose,blood lipid,blood viscosity and endothelin(ET)and other active factors were significantly reduced.Conclusion:(1)treatment of red light therapy can improve blood rheology,improve glucose and lipid metabolism,improve insulin sensitivity,Vascular endothelial cell activation and so on to improve the wound healing rate of DF wound in rats.(2)The treatment of red light therapy is effective,safe and high in clinical application.