糖尿病足溃疡组织中P物质表达量与氧化应激、细胞凋亡的相关性研究

The correlation of substance P expression in diabetic foot ulcer tissue with oxidative stress and apoptosis

目的:研究糖尿病足溃疡组织中P物质(SP)表达量与氧化应激、细胞凋亡的相关性。方法:选择在本院接受治疗的糖尿病足溃疡患者作为研究的糖尿病足溃疡(DFU)组并收集糖尿病足溃疡组织标本,同期因外伤在华中科技大学同济医学院附属武汉中心医院接受清创手术的患者作为研究的对照组并收集正常创面组织标本。检测创面标本中SP、氧化应激分子、细胞凋亡分子的含量。结果:DFU组糖尿病足溃疡创面标本中SP、Prdx6、GSHPX、SOD的含量显著低于对照组的正常创面标本,MDA、AOPP、Cyto-C、Caspase-9、Fas、FasL、Caspase-8、Caspase-3的含量显著高于对照组的正常创面标本;高SP患者创面中Prdx6、GSHPX、SOD的含量显著高于低SP患者,MDA、AOPP、Cyto-C、Caspase-9、Fas、FasL、Caspase-8、Caspase-3的含量显著低于低SP患者。结论:糖尿病足溃疡组织中SP的低表达能够激活氧化应激反应和细胞凋亡来参与溃疡创面的形成。

Objective:To study the correlation of substance P(SP)expression in diabetic foot ulcer tissue with oxidative stress and apoptosis.Methods:Patients with diabetic foot ulcer who were treated in the Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology between January2016and March2017were selected as the diabetic foot ulcer(DFU)group of the research,the diabetic foot ulcer tissue samples were collected,patients who received debridement surgery due to trauma in the Central Hospital of Wuhan,Tongji Medical College,Huazhong University of Science and Technology during the same period were selected as the control group of the research,and normal wound tissue samples were collected.The contents of SP,oxidative stress molecules and apoptosis molecules in the wound specimens were detected.Results:SP,Prdx6,GSHPX and SOD contents in diabetic foot ulcer wound specimens of DFU group were significantly lower than those in normal wound specimens of control group while MDA,AOPP,Cyto C,Caspase9,Fas,FasL,Caspase8and Caspase3contents were significantly higher than those in normal wound specimens of control group;Prdx6,GSHPX and SOD contents in wound of patients with high SP were significantly higher than those of patients with low SP while MDA,AOPP,Cyto C,Caspase9,Fas,FasL,Caspase8and Caspase3contents were significantly lower than those of patients with low SP.Conclusion:The low expression of SP in diabetic foot ulcer tissue can activate oxidative stress response and apoptosis to participate in the formation of ulcer wound.