穴位贴敷对变应性鼻炎大鼠IL-4、LTC4的影响

Impact of Acupoint Application on IL-4 and LTC4 in Rats with Allergic Rhinitis

目的:观察采用本院治未病贴膏进行穴位贴敷治疗对AR模型大鼠的治疗效应及对IgE、IL-4、LTC4的影响。方法:选用40只SPF级健康SD大鼠,盐水对照组、模型组、贴敷治疗组、丙酸氟替卡松组,采用OVA与氢氧化铝腹腔注射及OVA滴鼻诱发制备AR模型,盐水对照组予以同样剂量的生理盐水。观察大鼠鼻部症状,记录嗜酸性粒细胞数,检测血IgE、IL-4水平及鼻腔灌洗液LTC4表达。结果:1.与对照组比较,模型组大鼠鼻黏膜组织中EOS数量显著上升(P<0.01);穴位贴敷组、丙酸氟替卡松组与模型组比较,EOS数量显著降低(P<0.01);2.与盐水对照组比较,模型组大鼠外周血清IgE、IL-4含量显著升高(P<0.05);与模型对照组比较,贴敷治疗组与丙酸氟替卡松对照组血清IgE、IL-4含量均显著降低(P<0.05);3.与盐水组比较,模型组大鼠鼻腔灌洗液中的LTC4含量明显增高(P<0.01);模型组比较,穴位贴敷组鼻腔灌洗液中的LTC4含量减低(P<0.05)。结论:采用治未病贴膏穴位贴敷治疗可调节IgE、IL-4、LTC4水平,显著改善AR大鼠变应性反应。

Objective:To study the effect of acupoint application therapy with Zhiweibing(preventive treatment of disease)patch on AR(allergic rhinitis)rats,and its influence to serum IgE,IL-4and LTC4.Methods:40SD rats were randomly divided into the blank control group,AR model group,acupoint application group,and fluticasone propionate group.AR models were established by injecting OVA and aluminium hydroxide,and nasal inhalation of OVA.The blank control group was given normal saline.Nasal symptoms,eosinophils(EOS)amount,levels of IgE and IL-4,and expression of LTC4were observed and tested.Results:In terms of EOS amount,it was significantly increased in the model group compared to the blank control group(P<001);and it was decreased greatly after the treatment(P<001);in terms of serum IgE and IL-4,they were both became higher after modeling(P<0.05),and they were reduced obviously in the acupoint application group and fluticasone propionate group(P<0.05);in terms of LTC4expression,it was increased significantly in the model group compared to the blank control group(P<0.01),it was decreased greatly in the acupoint application group compared to the model group.Conclusion:Acupoint application can regulate IgE,IL-4and LTC4to improve allergic reaction in AR rats.