氨甲酰促红细胞生成素对糖尿病大鼠视网膜病理改变的影响

Effect of carbamylated erythropoietin on retinopathy of diabetic rats

目的:研究氨甲酰促红细胞生成素(CEPO)对糖尿病大鼠视网膜病理改变的影响。方法:选择雄性SD大鼠作为实验动物并随机分为对照组、DM组、CEPO组,建立糖尿病动物模型后给予CEPO干预。干预后2周,采集视网膜并检测血管新生分子、细胞凋亡分子、氧化应激通路分子的表达量。结果:DM组大鼠视网膜中HIF-1α、VEGF、Ang-1、Bax、Caspase-3、Nrf-2、ARE、HO-1、NQO-1的mRNA表达量显著高于对照组,TKLK、PEDF、Bcl-2、Survivin的mRNA表达量显著低于对照组;CEPO组大鼠视网膜中HIF-1α、VEGF、Ang-1、TKLK、PEDF的mRNA表达量与DM组比较无显著性差异,Bcl-2、Survivin、Nrf-2、ARE、HO-1、NQO-1的mRNA表达量显著高于DM组,Bax、Caspase-3的mRNA表达量显著低于DM组。结论:CEPO能够减轻糖尿病大鼠视网膜组织的细胞凋亡及氧化应激损伤,同时不影响血管新生过程。

Objective:To study the effect of carbamylated erythropoietin(CEPO)on retinopathy of diabetic rats.Methods:Male SD rats were selected as experimental animals and randomly divided into control group,DM group and CEPO group,and diabetic animal models were established and then given CEPO intervention.Two weeks after intervention,the retina was collected to detect the expression of angiogenesis molecules,apoptosis molecules and oxidative stress pathway molecules.Results:HIF1α,VEGF,Ang1,Bax,Caspase3,Nrf2,ARE,HO1and NQO1mRNA expressions in retina of DM group were significantly higher than those of control group,while TKLK,PEDF,Bcl2and Survivin mRNA expressions were significantly lower than those of control group;HIF1α,VEGF,Ang1,TKLK and PEDF mRNA expressions in retina of CEPO group were not significantly different from those of DM group,Bcl2,Survivin,Nrf2,ARE,HO1and NQO1mRNA expressions were significantly higher than those of DM group,and Bax and Caspase3mRNA expressions were significantly lower than those of DM group.Conclusion:CEPO can reduce the apoptosis and oxidative stress injury of the retina tissue in diabetic rats without affecting the angiogenesis.