术前新辅助化疗对结肠癌组织中恶性分子表达及根治手术所致创伤程度的影响

Effect of preoperative neoadjuvant chemotherapy on the expression of malignant molecules in colon cancer tissue and the degree of trauma caused by radical operation

目的:研究术前新辅助化疗对结肠癌组织中恶性分子表达及根治手术所致创伤程度的影响。方法:选择在唐山市丰润区人民医院诊断为结肠癌的患者,随机分为接受XELOX方案新辅助化疗联合结肠癌根治术的XELOX组及接受单纯结肠癌根治术的对照组。采集手术切除的结肠癌组织并检测增殖、凋亡及侵袭基因的表达量,采集血清并检测肝肾功能指标、炎症因子的含量。结果:XELOX组患者手术切除结肠癌病灶中Rac1、PLD2、CHD1L、Snail、Vimentin、N-cadherin的mRNA表达量均显著低于对照组,MS4A12、ASPP2的mRNA表达量均显著高于对照组;手术后1天和3天时,两组血清中ALT、AST、β2-MG、Cys-C、sICAM-1、sVCAM-1、sTM、sE-selectin的含量无显著性差异。结论:术前新辅助化疗能够抑制结肠癌组织中增殖、凋亡及侵袭基因的表达,同时不会增加手术操作的创伤程度。

Objective:To study the effect of preoperative neoadjuvant chemotherapy on the expression of malignant molecules in colon cancer tissue and the degree of trauma caused by radical operation.Methods:Patients who were diagnosed with colon cancer in Fengrun People's Hospital between March2014and February2017were selected and randomly divided into the XELOX group who accepted XELOX neoadjuvant chemotherapy combined with radical operation for colon cancer and the control group who accepted radical operation for colon cancer alone.Surgically removed colon cancer tissue was collected to test the expression of proliferation,apoptosis and invasion genes,and serum was collected to detect the contents of liver and kidney function indicators as well as inflammatory factors.Results:Rac1,PLD2,CHD1L,Snail,Vimentin and N cadherin mRNA expression levels in surgically removed colon cancer lesions of XELOX group were significantly lower than those of control group while MS4A12and ASPP2mRNA expression levels were significantly higher than those of control group;serum ALT,AST,β2MG,Cys C,sICAM1,sVCAM1,sTM and sE selectin contents were not significantly different between the two groups of patients1day and3days after surgery.Conclusion:Preoperative neoadjuvant chemotherapy can inhibit the proliferation,apoptosis and invasion gene expression in colon cancer tissues without increasing the trauma of operation.