载IR-780与多柔比星温敏脂质体的制备与表征

Preparation and characterization of thermo-sensitive liposomes loaded with IR-780 and doxorubicin

目的制备同时包封IR-780和多柔比星(DOX)的温敏脂质体并进行表征。方法采用薄膜水化法及硫酸铵梯度法制备载IR-780和DOX温敏脂质体(DOX-IR-780thermo-sensitive liposome,DITSL)。采用Malvern激光粒度仪检测各脂质体的粒径、表面电位及多分散系数(PDI);采用激光诱导DITSL升温释药,检测各脂质体的释药特性。结果 IR-780和DOX同时被包封于温敏脂质体,成功制备得DITSL。IR-780和DOX的包封率分别为(94.47±8.57)%、(92.52±7.61)%;平均粒径(138.98±8.74)nm;略带负电位;PDI为0.32±0.02。激光0.8 W/cm2照射5min,温度最高升到54.2℃,DOX释药率达80.1%。结论 DITSL具有药物包封率较高、粒径大小适宜、光热转化效率高、温度敏感性好的优点,并可通过激光控制释药,为下一步光热-化疗联合治疗肿瘤的研究奠定了基础。

ObjectiveTo prepare and characterize a thermo sensitive liposomes co loaded with IR-780and doxorubicin(DOX).MethodsMembrane hydration method and ammonium sulfate gradient method were used to prepare IR-780/DOX thermo sensitive liposomes(DITSL).The particle size,zeta potential and polydispersity coefficient(PDI)of liposomes were measured by Malvern laser particle size analyzer,and the drug release characteristic of DITSL induced by laser was also detected.ResultsDITSL loading both IR-780and DOX was successfully prepared.The encapsulation efficiency of IR-780and DOX was(94.47±8.57)%and(92.52±7.61)%,respectively;the average particle size was(138.98±8.74)nm,with slight negative potential;the PDI was0.32±0.02.The drug release rate of DITSL was about80.1%after laser radiation(0.8W/cm2,5min)and the highest temperature of DITSL was54.2℃.ConclusionThe prepared DITSL has high drug encapsulation efficiency,appropriate particle size,high photo thermal conversion efficiency,good temperature sensitivity and laser induced thermal drug release property,which lays a foundation study for the combination treatment of tumors with photo thermal therapy and chemotherapy.