丁苯酞对窒息性心脏骤停大鼠早期心肺复苏的影响

Effect of butylphthalide on early cardiopulmonary resuscitation in asphyxia cardiac arrest rats

目的:探讨丁苯酞对窒息性心脏骤停大鼠早期心肺复苏的影响。方法:健康SD大鼠15只,采用随机数字表法分为假手术组、心脏骤停复苏组、丁苯酞干预组,各5只。监测各组大鼠血流动力学指标,采用免疫组织化学法检测炎症因子,采用流式细胞仪检测相关因子。结果:3组大鼠自主循环恢复后,随时间推移,心率、平均动脉压均呈现出明显增加态势(P<0.05~P<0.01),实验中不同时间点心率与平均动脉压增加幅度均为丁苯酞干预组>心脏骤停复苏组>假手术组(P<0.05~P<0.01)。3组大鼠实验20 min时肿瘤坏死因子α、白细胞介素-6、细胞黏附因子-1均较实验前增加(P<0.05),白细胞介素-10、超氧化物歧化酶均较实验前降低(P<0.05);实验20 min时肿瘤坏死因子α、白细胞介素-6和细胞黏附因子-1均为假手术组大鼠>心脏骤停复苏组>丁苯酞干预组(P<0.01);实验20 min时白细胞介素-10和超氧化物歧化酶为丁苯酞干预组>心脏骤停复苏组>假手术组(P<0.05~P<0.01)。结论:对窒息性心脏骤停大鼠实施早期心肺复苏时,丁苯酞可改善血流动力学指标,减轻炎症反应,能提高超氧化物歧化酶活性,抑制细胞黏附因子-1表达。

Objective:To investigate the effects of butylphthalide on early cardiopulmonary resuscitation in asphyxia cardiac arrest rats.Methods:Fifteen healthy SD rats were divided into the sham group,cardiopulmonary resuscitation group and butylphthalide intervention group using random number table method(5rats each group).The hemodynamic parameters were monitored,the inflammatory factors were detected suing immunohistochemistry,and the correlate factors were detected using flow cytometry.Results:After spontaneous circulation recovery in3groups,with the time passing,the heart rate and mean arterial pressure gradually increased(P<0.05to P<0.01).The increasing degrees of the heart rate and mean arterial pressure in butylphthalide intervention group were higher than those in cardiopulmonary resuscitation group,and the increasing degrees of the heart rate and mean arterial pressure in cardiopulmonary resuscitation group were higher than those in sham group(P<0.05to P<0.01).Compared with before experiment,the levels of the tumor necrosis factorα,interleukin6and cell adhesion factor1increased(P<0.05),while the levels of interleukin10and SOD decreased in three groups at20min of experiment(P<0.05).At20min of experiment,the levels of tumor necrosis factorα,interleukin6and cell adhesion factor1in sham group were more than those in cardiopulmonary resuscitation group,and the levels of tumor necrosis factorα,interleukin6and cell adhesion factor1in cardiopulmonary resuscitation group were more than those in butylphthalide intervention group(P<0.01).At20min of experiment,the levels of interleukin10and SOD in butylphthalide intervention group were more than those in cardiopulmonary resuscitation group,and the levels of interleukin10and SOD in cardiopulmonary resuscitation group were more than those in sham group(P<0.05to P<0.01).Conclusions:During the early cardiopulmonary resuscitation in asphyxia cardiac arrest rats,the butylphthalide can improve hemodynamics,reduce inflammation reaction,increase SOD activity and inhibit the expression of cell adhesion factor1.