医用聚乙烯表面接枝2-甲基丙烯酰氧乙基磷酸胆碱对细菌生物膜形成的影响

Effect of MPC coating medical polyethylene surface on bacterial biofilm formation

目的:探讨医用聚乙烯表面接枝2-甲基丙烯酰氧乙基磷酸胆碱(MPC)对金黄色葡萄球菌生物膜形成的影响。方法:利用紫外光接枝法将MPC接枝于医用聚乙烯薄片表面并用扫面电镜观察其表面形貌。以金黄色葡萄球菌(ATCC25923)标准菌株作为实验用菌,将已消毒的已接枝MPC聚乙烯薄片(观察组)及普通聚乙烯薄片(对照组)置入5 m L细菌浓度为1.5×107cfu/m L的菌液中共同培养。分别于体外培养后24 h、48 h行膜内活菌计数和扫描电镜观察。结果:扫描电镜观察显示通过紫外光接枝法制备的MPC涂层聚乙烯薄片表面形貌光滑,原先的纹理消失。体外培养24 h、48 h观察组聚乙烯薄片表面活菌计数数量均显著少于对照组(P<0.01);扫描电镜观察下进行细菌数半定量评估显示24 h、48 h观察组聚乙烯薄片表面细菌黏附量亦均少于对照组(P<0.01)。结论:医用聚乙烯表面接枝MPC可有效抑制细菌黏附,进而可预防聚乙烯表面细菌生物膜的形成。

Objective:To explore the effects of medical polyethylene surface coated by2methacryloyloxy ethyl phosphoric acid choline(MPC)on the formation of bacterial biofilm.Methods:The surface of medical polyethylene was coated with MPC using ultraviolet light grafting method,and its morphology was observed by scanning electron microscopy.The standard strain of Staphylococcus aureus(ATCC25923)was chose,and the polyethylene plate surface coated by MPC(observation group)and ordinary plate(control group)were co cultured with5mL bacterial liquid(1.5×107cfu/mL),respectively.After24h and48h of culturing,the viable bacteria adhered on the surface of plate in two groups were counted,and scanned by electron microscopy.Results:Scanning electron microscopy results showed that the medical polyethylene plate surface coated by MPC was smooth,the original texture of which disappeared.After24h and48h of culturing,the quantity of viable bacteria in observation group was significantly less than that in control group(P<0.05),and scanning electron microscopy results showed that the amount of adhesion bacterial of plate surface in observation group was significantly less than that in control group(P<0.05).Conclusions:MPC coating on medical polyethylene surface can effectively inhibit the bacterial adhesion,and prevent the bacterial biofilm formation.