马齿苋总黄酮对Aβ_(25-35)阿尔茨海默症小鼠学习记忆功能的影响

Effects of Total Flavone in Portulacaoleracea L. on Learning and Memory of Alzheimer's Disease Mice Caused by Aβ_(25-35)

本实验通过观察马齿苋总黄酮(POTF)对β-淀粉样蛋白(Aβ25-35)所致阿兹海默症(AD)模型小鼠学习记忆的影响,探讨马齿苋总黄酮对AD的改善作用。60只小鼠随机分组,采用海马组织注射Aβ25-35构建AD小鼠模型,经马齿苋总黄酮灌胃治疗30 d后,检测小鼠空间学习记忆能力、海马组织中乙酰胆碱(ACh)和乙酰胆碱酯酶(Ach E)活性、环磷腺苷效应元件结合蛋白(CREB)mRNA和蛋白的相对表达。结果显示,与模型组相比,POTF高剂量组小鼠空间学习记忆能力显著增加(P<0.01),海马组织ACh、Ach E和CREB蛋白的表达均明显上升(P<0.05)。因此,马齿苋总黄酮(POTF)可能通过增强海马组织胆碱能的代谢,增强CREB信号通路,改善Aβ25-35所致AD小鼠学习记忆能力。

This study was designed to investigate the effects of total flavone in Portulacaoleracea L.(POTF)on learning and memory of mice with Alzheimer’s disease(AD)caused by Aβ2535.Sixty mice were divided randomly into six groups:sham operation group,model group,high,medium and low dose of POTF treatment groups.AD mice were established by hippocampal injection of Aβ2535.Different concentrations of POTF were treated for30days.Morris water maze was used to test the spatial learning and memory ability in each group.Hydroxylamine and spectrophotometer were used for the activities of Ach and AchE in hippocampus,respectively.Expression of CREB mRNA and protein was detected by qPCR and Western blot.The results showed that compared with sham group,the spatial learning and memory in model group was significantly decreased(P<0.01),the levels of Ach,AchE and CREB were also decreased(P<0.05).Compared with model group,mice treated with high dose of POTF showed much less latency(P<0.01)and increased times across platform(P<0.01)in Morris water maze,and higher levels of Ach,AchE and CREB mRNA and protein in hippocampus in different degree(P<0.05or P<0.01).In conclusion,POTF has a protective effect on the learning and memory of AD mice by enhancing the metabolism of Ach and CREB pathway in hippocampus.