高效液相色谱法同时测定青钱柳中9种指标成分的含量

Simultaneous Determination of Nine Chemical Markers of Cyclocaryae Folium by HPLC

建立高效液相色谱法同时测定青钱柳中绿原酸、香草酸、逆没食子酸、异槲皮苷、阿福豆苷、槲皮苷、山奈酚、山奈素和乌苏酸含量的方法。采用Agilent ZORBAX Eclipse XDB-C18色谱柱(150 mm×4.6 mm,3.5μm);流动相为0.2%磷酸乙腈-0.2%磷酸水,梯度洗脱;流速为0.7 m L/min,柱温35℃;检测波长:香草酸、逆没食子酸和乌苏酸为210 nm;绿原酸、异槲皮苷、阿福豆苷、槲皮素、山奈酚和山奈素为360 nm。青钱柳在该色谱条件下9种成分分离较好,平均加样回收率为95.86%~103.30%,RSD<2.5%。该方法简便、准确、重复性好,适用于青钱柳中绿原酸、香草酸、逆没食子酸、异槲皮苷、阿福豆苷、槲皮苷、山奈酚、山奈素和乌苏酸含量的同时测定。

HPLC method was developed for the simultaneous determination of chlorogenic acid,vanililic acid,ellagic acid,sioquercitin,afzelin,quercetin,kaempferol,kaempferidel and ursolic acid in Cyclocaryae Folium.The ZORBAX Eclipse XDB C18column(150mm×4.6mm,3.5μm)was used with0.2%phosphoric acid in acetonitrile(A)and02%phosphoric acid in water(B)as mobile phases in gradient elution mode.The flow rate was0.7mL/min,the column temperature was35℃.The DAD detection wavelength was set at210nm for vanililic acid,ellagic acid and ursolic acid,360nm for the others.As a result,nine chemical markers were separated well,the average recoveries were between9586103.30%,RSD<2.5%.The developed HPLC method was simple,accurate and reproductive.It is suitable for the determination of nine chemical markers of Cyclocaryae Folium.