产红色素真菌Monascus sanguineus的分离及其色素提取条件研究

Isolation and Extraction Optimization of Red Pigment from Monascussanguineus

采用组织块分离法首次从药用植物地黄中分离出一株产红色素内生真菌RJL03,根据形态学特征和r DNA ITS1-5.8S-ITS2序列分析的手段鉴定为Monascus sanguineus。采用响应面法(RSM)对菌株RJL03中红色素提取工艺条件进行研究。在单因素实验的基础上,以提取液OD值为指标,利用Box-Behnken中心组合实验设计原理(BBD)优化菌株中红色素的有机溶剂超声波辅助浸提(UAE)工艺。结果显示,该色素提取液在515 nm处有最大吸收波长,最佳提取工艺为温度70℃、提取时间34 min、乙醇浓度50%。在此条件下提取菌株中的红色素,得到的提取液OD值为2.587,可比优化前提高约1.5倍。

A strain of red pigment fungi RJL03was isolated from the medicinal plant Rehmannia glutinosa Libosch by tissue block separation method.Monascus sanguineus was identified according to morphological characteristics and rDNA ITS15.8S ITS2sequence analysis.The extraction process conditions of RJL03red pigment were explored by Response Surface Methodology(RSM).On the basic of single factor tests,the organic solvent Ultrasonic Assisted Extraction(UAE)process of red pigment in the strain was optimized using the Box Behnken design(BBD),with the optical density of the extract as an index.The experiment manifests that the extraction of pigment has its maximum absorption wavelength at515nm.The optimal extraction temperature was70℃,the extraction time was34min,and the ethanol concentration was50%(V/V).Under these conditions,the red pigment in the strain was extracted after fermentation,and comparable to the amount of pre optimized extraction increased by about1.5times.