中晚期宫颈鳞状细胞癌同步放化疗敏感性相关血清miRNA的临床研究

The clinical study on simultaneous chemoradiotherapy sensitivity related serum miRNA of middle and advanced cervical squamous cell carcinoma

目的探讨中晚期宫颈鳞状细胞癌同步放化疗敏感性相关的血清miRNA。方法收集2014年2~12月在我院妇科接受根治性同步放化疗的20例中晚期宫颈癌患者为研究对象,按照患者治疗过程及随访过程中肿瘤评估的情况,将研究对象分为同步放化疗抗拒组(n=10)和同步放化疗敏感组(n=10),筛选抗拒组和敏感组间具有显著表达差异的miRNA,对比两组mi R-136-5p、mi R-152-3p、mi R-206的表达情况。结果在芯片筛选出的miRNA中,抗拒组和敏感组之间具有显著表达差异的miRNA有3个:抗拒组相较敏感组低表达的为mi R-136,抗拒组相较敏感组高表达的为mi R-152和mi R-206。标本定量验证结果显示,敏感组hsa-mi R-136-5p的表达水平高于抗拒组,差异有统计学意义(P<0.01);敏感组hsa-mi R-206的表达水平低于抗拒组,差异有统计学意义(P<0.01);敏感组和抗拒组hsa-mi R-152-3p的表达水平,差异无统计学意义(P>0.05)。结论中晚期宫颈癌患者血清中miRNA-136的表达下调、miRNA-206的表达上调可能与同步放化疗抗拒有关。

Objective To investigate the simultaneous chemoradiotherapy sensitivity related serum miRNAs of middle and advanced cervical squamous cell carcinoma.Methods A total of20patients with middle and advanced cervical squamous cell carcinoma who underwent radical concurrent radiotherapy and chemotherapy from February2014to December2014were enrolled in this study.According to the course of treatment and the evaluation of tumor during follow-up,the subjects were divided into simultaneous chemoradiotherapy resistant group(n=10)and simultaneous chemoradiotherapy sensitive group(n=10).The miRNAs with significant difference in expression between the resistant group and the sensitive group were screened.The expressions of miR-136-5p,miR-152-3p and miR-206were compared between the two groups.Results In the miRNA selected by chip,there were three miRNAs with significant difference between the resistant group and the sensitive group.The expression of miR-136was lower in the resistant group than that in the sensitive group,and the expressions of miR-152and miR-206were higher than those of the sensitive group.Specimen quantitative verification results showed that the expression level of miR-136-5p in sensitive group was higher than that in resistant group,and the difference was significant(P<0.01).The expression level of miR-206in sensitive group was lower than that in resistant group,and the difference was statistically significant(P<0.01).There was no significant difference in the expression level of miR-152-3p between sensitive group and resistant group(P>0.05).Conclusion The downregulation of miRNA-136and upregulation of miRNA-206may be related to the resistance of the simultaneous chemoradiotherapy in patients with middle and advanced cervical squamous cell carcinoma.