离子型稀土矿山集液沟区域耐盐异养硝化细菌的筛选及其脱氮特征

Screening salt tolerant heterotrophic nitrifying bacteria from the collecting groove region in ion-type rare earth mines and its nitrogen removal characteristics

为筛选处理高盐含氨氮矿冶废水的耐盐微生物,从赣南离子型稀土矿山集液沟区域采集样品,利用选择性培养基富集培养分离耐盐异养硝化细菌,并考察碳源、pH、盐度等因素对其脱氮性能的影响.结果表明,分离得到一株耐盐异养硝化细菌X1,经16S rDNA鉴定为肠杆菌属Enterobacter sp.X1,确定该菌适宜的脱氮条件为:以蔗糖作为碳源,pH值为5,盐度≤15%.菌株X1的NH_4^+-N去除率可达到60%以上.研究结果可为高盐含氨氮矿冶废水的微生物处理提供菌株和数据参考.

To screen the salt tolerant microorganism with high salinity and ammonia nitrogen mining and metallurgy wastewater,the samples were collected from the collecting groove region of ion-type rare earth mines in south of Jiangxi province,and the salt tolerant heterotrophic nitrifying bacteria were cultured and isolated by selective medium.Apart from that,the effect of carbon source,pH,and salinity on its nitrogen removal performance was investigated.The results show that a salt tolerant heterotrophic nitrifying bacterium X1is isolated and identified as Enterobacter sp.X1by16S rDNA analysis.The suitable condition for nitrogen removal is determined as follows:the sucrose is the carbon source,the pH value equal to5,and the salinity less than or equal to15%.The removal rate of NH4+-N of strain X1could reach above60%.These results could provide a reference for the microbial treatment of wastewater with high salt and ammonia nitrogen.