烟草青枯菌FQY_4的抗生素类抗/耐药性相关基因分析

Antibiotics Resistance Genes on Ralstonia solanacearum FQY_4

为探明烟草青枯菌基因组中抗/耐药性基因作用机理。在烟草青枯菌FQY_4基因组测序的基础上,结合培养青枯菌的半选择性培养基中抗生素的使用,采用基因注释及同源基因比较法,分析青枯菌FQY_4基因组携带的抗/耐药性相关基因。结果显示:青枯菌FQY_4染色体上和巨大质粒上分别有6个和12个与多抗/耐药性相关的基因,包括多粘菌素耐药蛋白基因、膜融合蛋白CmeA基因、二甲基腺苷转移酶基因、膦胺霉素耐药性蛋白基因、外膜多耐药性系统相关基因和抗四环素基因序列。

In order to explore the function mechanism of resistance genes in the genome of R.solanacearum,based on the sequenced genome of FQY_4,according to the antibiotics used in semiselective medium SMSA for R.solanacearum,the resistance genes on chromosome of FQY_4 strains were analyzed through the methods of genome comparative and homology blast.Results:There are six resistance genes on chromosome of FQY_4 and 12 resistance genes on large plasmid of FQY_4,including polymyxin resistance protein,membrane fusion protein CmeA,tripartite multidrug resistance system,fosmidomycin resistance protein and tetracycline resistance sequences,which related to the antibiotics resistance.