猕猴急性脊髓全横断损伤血清的代谢组学分析

Serum metabolomic profiling of acute complete spinal cord transection in macaques

背景:脊髓损伤通过非靶标代谢组学分析可用于发现和识别代谢物变化,分析其与脊髓损伤的关系,有助于理解并进一步探讨继发脊髓损伤的病理生理过程和机制。目的:检测分析猕猴脊髓全横断损伤后血清代谢物变化,探讨其与脊髓损伤病理生理过程的关系,筛选潜在生物标志物。方法:对5只成年猕猴脊髓全横断损伤前1 d(正常对照),损伤后3 h(超急性期)及3 d(急性期)血清进行气相飞行时间质谱(GC/TOF-MS)非靶标检测,通过与质谱图库比对,指认代谢产物,查找差异代谢物,并映射至相关代谢通路,分析病理生理过程和作用机制。结果与结论:质谱分析共识别出358个谱峰,14个代谢物被认定为差异代谢物,包括小分子有机酸,氨基酸,脂肪酸和糖。结果显示,全横断脊髓损伤急性期与氨基酸代谢、三羧酸循环和丙酮酸代谢密切相关。

BACKGROUND:Non-targeted metabolomic profiling is used to uncover metabolic changes and to identify relationships between metabolites and spinal cord injury,which contributes to further understanding the pathophysiological process and mechanisms of secondary spinal cord injury.OBJECTIVE:To detect and analyze the serum metabolite changes after complete spinal cord transaction in macaques,explore its relationship with the pathophysiological progress of spinal cord injury,and screen the potential biomarkers.METHODS:Five adult macaques were selected,in which the models of complete spinal cord transaction were established.The serum metabolic features were detected using a non-targeted metabolic profiling strategy based on gas chromatography time-of-flight mass spectrometry at1day before modeling,3hours(superacute phase)and3days(acute phase)after modeling.After compared with the spectrometry profiling,recognizing the metabolites,searching for differential metabolites and the related metabolic pathways,the pathophysiological process and mechanisms were analyzed.RESULTS AND CONCLUSION:Three hundred and fifty-eight chromatographic peaks were obtained for subsequent data analysis.Fourteen metabolites,including low-molecular-weight organic acid,amino acids,fatty acid and carbohydrate,were identified as differential metabolites.To conclude,the acute phase of complete spinal cord transection is closely related to some metabolic pathways,such as amino acid metabolism,tricarboxylic acid cycle and pyruvate metabolism.