高效液相色谱法同时测定痤疮消炎洗剂中黄芩苷蒙花苷大黄酸含量

Simultaneous Determination of Baicalin,Linarin,Rhein in Cuochuang Xiaoyan Lotion by HPLC Method

目的建立同时测定痤疮消炎洗剂中黄芩苷、蒙花苷、大黄酸含量的高效液相色谱(HPLC)方法。方法采用高效液相-梯度洗脱法测定痤疮消炎洗剂中3种成分含量,色谱柱为Waters XTerra MS C18(4.6 mm×250 mm,5μm);乙腈为流动相A,0.2%磷酸溶液为流动相B(调节pH值2.0),梯度洗脱流速1.0 m L·min-1,检测波长277 nm,柱温30℃。结果样品中3种成分与相邻色谱峰完全分离,黄芩苷、蒙花苷、大黄酸进样浓度与峰面积呈良好的线性关系,线性方程为:Y黄芩苷=9.208X黄芩苷-0.099 4(R2=0.999 9,浓度范围83.97~839.70μg·m L-1);Y蒙花苷=3.062 8X蒙花苷-0.003 8(R2=0.999 9,浓度范围34.75~347.49μg·m L-1);Y大黄酸=1.022 5X大黄酸-0.028 6(R2=0.999 8,浓度范围63.20~632.00μg·m L-1)。结论所建立的HPLC测定方法,样品制备方法简便,重复性好,为制定痤疮消炎洗剂的质量标准提供了可靠的含量测定方法。

ObjectiveTo establish a specific HPLC method for simultaneous determination of three components(baicalin,linarin and rhein)in Cuochuang Xiaoyan lotion.MethodsThe three components in Cuochuang Xiaoyan lotion were assayed by HPLC gradient elution method.The assay was performed with Waters XTerra MS C18(4.6mm×250mm,5μm)column,with acetonitrile(A)and0.2%phosphoric acid solution(B)as mobile phase in gradient elution.The flow rate was1.0mL·min-1.The detection wave length was277nm and column temperature was30℃.ResultsThree components were completely separated from the adjacent peaks and a good linear relationship between each sample concentration and integral area was obtained.The linear equations were as follows:YBaicalin=9.208X-0.0994(R2=0.9999,83.97-839.70μg·mL-1);YLinarin=3.0628X-0.0038(R2=0.9999,34.75-347.49μg·mL-1);YRhein=1.0225X-0.0286(R2=0.9998,63.20-632.00μg·mL-1).ConclusionThe HPLC method is simple,accurate and reproducible,which is effective in controlling the quality of Cuochuang Xiaoyan lotion.