摘要
目的探讨积雪草苷对博莱霉素诱导的小鼠皮肤瘢痕形成的抑制作用及其可能的作用机制。方法雄性C57BL/6小鼠30只,随机分为阴性对照组、模型对照组以及积雪草苷组,每组10只,模型对照组和积雪草苷组小鼠每日于背部经皮下注射博莱霉素(1 mg·mL^(-1))1 mL,积雪草苷组于4 h后经皮下注射积雪草苷(20 mg·mL^(-1))1 mL,模型对照组给予相同剂量的0.9%氯化钠溶液,阴性对照组则在此两个时间点给予相同剂量0.9%氯化钠溶液,21 d后处死全部小鼠。进行皮肤组织形态学观察、肌成纤维细胞增殖以及炎症因子表达的检测。结果病理组织学显示,积雪草苷组皮肤瘢痕较薄,纤维化程度较模型对照组明显减轻,其真皮层胶原厚度显著小于模型对照组厚度;皮肤纤维化程度指标α-平滑肌肌动蛋白的表达也明显低于模型对照组,并且炎症细胞浸润减少。结论积雪草苷可抑制博莱霉素诱导的小鼠皮肤瘢痕的形成,其机制可能与调节肌成纤维细胞以及炎症细胞的表达相关。
ObjectiveTo evaluate the inhibitory effect of asiaticoside on bleomycin induced skin cicatrization.MethodsThirty male C57BL/6mice were randomly divided into three groups:negative control group,model control group,and asiaticoside group,ten in each group.In model control group and asiaticoside group,1mg·mL-1bleomycin was subcutaneously injected into the dorsal skin of mice every day;4h later,1mL0.9%sodium chloride solution1mL asiaticoside(20mg·mL-1)was injected into the lesion skin in the model control group and the asiaticoside group,respectively.In the negative control group,the same volume of0.9%sodium chloride solution was subcutaneously injected into the dorsal skin of the mice at the two time points every day.After21days,skin specimens were harvested to observe the histomorphology and detect myofibroblast proliferation and expression of inflammatory factors.ResultsThe skin scar was significantly attenuated in the asiaticoside group as compared with the model control group,and the dermal thickness measured exhibited a gradual decrease in asiaticoside group.The expression ofαantismooth muscle antisbidy and infiltration of inflammatory cells were significantly lower in the asiaticoside group than in the model control group.ConclusionAsiaticoside inhibits the development of skin scar of mice by regulating proliferation and differentiation of myofibroblasts and down regulating inflammatory cells.
作者
王莎丽
杨琳
刘勇
姚超然
宋爽
刘洋
袁晓环
武艳
WANG Shali;YANG Lin;LIU Yong;YAO Chaoran;SONG Shuang;LIU Yang;YUAN Xiaohuan;WU Yan(Grade 2012,Pharmacy Department Mudanjiang Medical College,Mudanjiang 157011,China;Medical Research Center,Mudanjiang Medical College,Mudanjiang 157011,China)
出处
《医药导报》
CAS
2017年第12期1363-1366,共4页
Herald of Medicine
基金
国家级大学生创新创业训练计划项目(201510229012)
牡丹江医学院科学技术研究项目(ZS201519)
黑龙江省普通高校组织损伤与修复重点实验室科学技术研究项目(zdsys 2012-10)