基于指纹图谱与一测多评技术分析化学转化法富集甘草药渣总黄酮的研究

Study on Enrichment of Total Flavonoids from Licorice Residue by Chemical Conversion Method Based on Fingerprint and Quantitative Analysis of Multi-components with a Single-marker Technique

目的建立指纹图谱与一测多评相结合的质量评价模式分析化学转化法富集的甘草药渣总黄酮,为生产中质量控制提供技术支撑。方法以破壁富集的总黄酮为研究对象建立指纹图谱,以甘草苷为内参物,分别建立异甘草苷、甘草素、异甘草素、甘草酸的相对校正因子,计算其含量。并对计算值与外标法测定值进行比较,以验证一测多评法的实用性与稳定性。结果建立了甘草药渣总黄酮HPLC指纹图谱,标定了11个共有峰,确定了其中5个共有峰,10批提取物相似度>0.99;一测多评法计算结果与外标法实测值之间相对误差<4%,以多浓度法计算试验所得相对校正因子RSD<2%。结论本方法准确可靠,专属性强,结果稳定,重复性好,可有效控制甘草药渣提取物的质量。

Objective To establish a combined quality evaluation model of fingerprint and quantitative analysis of multi-components with a single-marker(QAMS)to analyze the total flavonoids from licorice residue by the chemical conversion method;To provide technical support for quality control in production.Methods Total flavonoids of breaking cell wall and enriching were taken as the object of study to establish fingerprint.With liquiritin as internal reference,the relative correction factors of isoliquiritin,glycyrrhizin,isoliquiritigenin and glycyrrhizic acid were established respectively,and the contents were determined.Meanwhile,the calculated values were compared with the measured value by external standard method to verify the practicability and stability of QAMS.Results The HPLC fingerprint of total flavonoids from licorice residue was established.11common peaks were identified,and5common peaks were identified,and the similarity of the10extracts was>0.99;the relative error between the calculated results of QAMS and the measured values of the external standard method was<4%;the RSD of relative correction factor calculated by the multiple concentration method was<2%.Conclusion The method is accurate,reliable,specific,and stable,with good repeatability,which can be used for the quality control of total flavonoids from licorice residue.