大孔吸附树脂纯化枳椇叶中总黄酮工艺研究

Study on the Purifying Technology of the Total Saponins in Hovenia Dulcis by Macroporous Resin

目的:建立大孔树脂分离纯化枳椇叶子中总黄酮的工艺。方法:通过比较9种大孔树脂对枳椇叶提取液的静态吸附率和解析率,优选一种,进一步考察其分离枳椇叶总黄酮的吸附性能和洗脱参数。结果:优选LSD-958大孔树脂,采用本实验确定的工艺参数枳椇叶总黄酮的纯度可由原样的10.14%提升到51.56%,纯化效果较好。结论:LSD-958大孔吸附树脂可以对枳椇叶中的黄酮类物质起到很好的"去粗取精"的作用。。

Objective:To study the separation and purification process of total flavone in hovenia dulcis leaves with macropore resin.Methods:Through the comparison of9kinds of macroporous resins hoveniaacerbalindl leaf extract static adsorption rate and desorption rate,choose the best to further study its adsorption properties and elution parameters.Result:Optimization of LSD-958macroporous resin,total flavone of hovenia dulcis leaves purity can increase by10.14%to51.56%,better purification effect.Conclusion:LSD-958macroporous adsorption resin can play a good role in“de-fining”of flavonoids in Hovenia dulcis Thunb leaves.