固相萃取法测定食品中胭脂树橙的含量

Determination of Carmine in Food by Solid Phase Extraction

胭脂树橙是从胭脂树种子中制取的一种天然食用色素。根据提取条件的不同,胭脂树橙主要分为油溶性和水溶性两种。油溶性胭脂树橙主要成分为红木素,水溶性胭脂树橙主要成分为降红木素。胭脂树橙是一种重要的食用天然色素,在国内外食品工业上受到广泛的应用,也是我国GB 2760-2014《食品安全国家标准食品添加剂使用标准》中规定的可以在相应产品中限量添加的食用色素。本文建立了测定食品中胭脂树橙含量的高效液相色谱法,采用氨水乙醇溶液提取,提取液经固相萃取净化后,供高效液相色谱测定。方法的定量限为0.5 mg/kg,回收率为83.17%~93.36%,相对标准偏差为2.28%~6.54%。该方法补充和改进了食品添加剂检测方法,为江苏食品安全质量的监管提供了技术支撑,有助于严厉打击及有效控制不法厂商的违法行为。

Annatto is a natural food coloring ingredient derived from the seeds of the tropical shrub(Bixa orellana L.).Depending on the extraction conditions,annatto can be divided into two main components:bixin and norbixin.The main ingredient of oil-soluble annatto is bixin,while the main component of water-soluble annatto is norbixin.As an important edible natural pigment,it’s widely used in food industry at home and abroad.Annatto can be added into specified food products according to GB2760-2014,national food safety standards of using food additives.Our project established a high performance liquid chromatography,method applied to the determination of annatto in food.The sample was extracted by ammonia-ethanol solution,then the extracted liquid was purified by solid phase extraction and determined by HPLC.The quantitative limit of the method was0.5mg/kg,the recovery ranged from83.17%to93.36%,and the relative standard deviation was2.28%~6.54%.The supplementary and improved food additives detection method can provide technical support for Jiangsu food safety and quality supervision,helping crack down upon the illegal actions of illegal manufacturer and effectively control them.