不同代次蛹虫草菌种在两种培养方式下的产量及遗传稳定性

Yield and Genetic Stability of Different Generations of Cordyceps militaris Under Two Ways of Cultivation

人工培养蛹虫草是解决野生虫草来源不足的良好途径,但是人工栽培子实体过程高代次菌种退化现象是制约产业化开发的一大瓶颈。分离纯化蛹虫草菌种并连续传代,同一代次菌种分别进行子实体培养和菌丝体培养,以长势和产量为检测指标,考察不同代次菌种在不同培养方式下的产量及遗传稳定性,筛选一种不受菌种代次影响的培养方式。结果表明:多次传代(6次传代)菌株的子实体培养有明显退化现象,但菌丝体培养不受菌种代次影响,且传代过程遗传稳定,无遗传差异。菌丝体培养具有应用于工业生产的良好前景。

Artificial culture Cordyceps militaris is the well way to solve the insufficient resource of wild Cordyceps.In the process of artificial culture Cordyceps militaris,the degeneration phenomenon of the strains is the restrictive bottleneck of the industrial exploitation.Isolation and purification of Cordyceps militaris strains and continuous passage,the identical strain was cultured in two methods:sporocarp and mycelium culture.Based on the test index of growth potential and yield,the yield and test genetic stability of the identical strain under different culture methods were studied,in order to screen a culture method without the effect of strains generation.The results showed that after six generations,the strains grew well and inherited stably in mycelium culture,although they obviously degenerated in sporocarp culture.Mycelium culture has a good prospect in industrial production.