黄芪皂甙Ⅳ在C57BL/6小鼠肾脏缺血再灌注损伤中的作用

Role of astragaloside Ⅳ on renal ischemia reperfusion injury in C57BL/6 mice

目的:探讨黄芪皂甙Ⅳ对C57BL/6小鼠肾脏缺血再灌注损伤的作用。方法:将24只SPF级C57BL/6小鼠随机分为四组:对照组(C组)、阴性治疗组(S组)、肾脏缺血再灌注组(I组)、肾脏缺血再灌注损伤治疗组(IA组)。肾脏缺血再灌注损伤模型采用双侧肾动脉夹闭的方法制作。四组小鼠均分离双侧肾动脉,C组:尾静脉给予生理盐水;S组:尾静脉给予黄芪皂甙Ⅳ(2mg/kg);I组:夹闭双层肾动脉,尾静脉给予生理盐水;IA组:夹闭双侧肾动脉,尾静脉给予黄芪皂甙Ⅳ(2mg/kg)。生理盐水和黄芪皂甙Ⅳ每天1次,连续给予7天。用肾功能检测、组织损伤评分、Western blot法检测PUMA的表达,ELISA法检测肾组织TNF-α、IL-6的表达。结果:与C组、S组比较,I组小鼠血清BUN、Scr、炎症因子TNF-α、IL-6及肾组织中PUMA均明显升高(P<0.05);经黄芪皂甙Ⅳ预处理,与I组比较,IA组中小鼠血清中BUN、Scr、炎症因子TNF-α、IL-6及肾组织中PUMA均明显降低(P<0.05)。结论:黄芪皂甙Ⅳ通过阻止PUMA上调和TNF-α、IL-6的表达来保护肾脏缺血再灌注损伤。

Objective:To investigate the role of astragalosideⅣon renal ischemia reperfusion injury in C57BL/6mice.Methods:24SPF C57BL/6mice were randomly divided into four groups:the control group(group C),negative treatment group(group S),renal ischemia reperfusion group(group I),renal ischemia reperfusion injury treatment group(group IA).Renal ischemia reperfusion injury model was made by clipping bilateral renal artery.Bilateral renal artery of mice was separated.Physiological saline was injected through the caudal vein in group C;astragalosideⅣ(2mg/kg)was injected through the caudal vein in group S;bilateral renal artery was clipped and physiological saline was injected through the caudal vein in group I;bilateral renal artery was clipped and astragalosideⅣ(2mg/kg)was injected through the caudal vein in group IA.Physiological saline and astragalosideⅣwere injected,1time a day,7days in a row.Renal function was measured,tissue injury was scored,expression of PUMA was measured by western blot,TNF-αand IL-6was measured by ELISA.Results:Compared with group C and group S,BUN,Scr,TNF-αand IL-6in serum and PUMA in renal tissue were increased in group I(P<0.05);after treated with astragalosideⅣ,compared with group I,BUN,Scr,TNF-αand IL-6in serum and PUMA in renal tissue were decreased in group IA(P<0.05).Conclusion:AstragalosideⅣprotected renal ischemia reperfusion injury by inhibiting the expression of PUMA,TNF-α,and IL-6.