摘要
目的:探讨肾宁Ⅰ号合剂对糖尿病肾病(DN)大鼠调节性Treg细胞的调节作用。方法:选取雄性SD大鼠50只,正常组10只,造模组40只采取高脂饮食联合低剂量链脲佐菌素(STZ)腹腔注射建立糖尿病肾病模型,将成模大鼠随机分为模型组、氯沙坦组、肾宁I号合剂低剂量组及肾宁I号合剂高剂量组,每组10只,后三组予相应药物灌胃,模型组予生理盐水灌胃,6周后留取标本,流式细胞术测定外周血Treg细胞水平。结果:DN大鼠尿白蛋白肌酐比值(UACR)显著高于正常组,肾小球系膜细胞及系膜基质增多,肾宁I号合剂可以减少UACR,效果与氯沙坦无差异,肾宁I号合剂低剂量优于高剂量;与肾宁I号合剂相比,氯沙坦有增加肾损伤风险,肾宁I号合剂高剂量组血肌酐明显低于低剂量组;在缓解肾脏病理方面肾宁I号合剂优于氯沙坦。DN大鼠外周血Treg比例下降,肾宁I号合剂及氯沙坦可以调整Treg失衡,但二者无统计学差异。结论:肾宁I号合剂是治疗DN的安全有效方剂。肾宁I号合剂可能通过调节Treg细胞,抑制DN免疫炎症反应,缓解DN病变。
Objective:To investigate the effects of the Shenning mixtureⅠon Treg cells of diabetic nephropathy rats.Methods:50SD rats were divided into the normal group(10rats)and modeling group40rats,raised with high fat diet,intraperitoneally injected low dose of STZ to establish diabetic nephropathy models)which were randomly divided into the model group,losartan group,Shenning mixture I low dose group and Shenning mixture I high dose group,10rats in each group.Rats in the last three groups were treated with corresponding drugs by gavage;the model group was given saline lavage.Specimens were obtained in after6weeks;the level of Treg cells in peripheral blood was determined by flow cytometry.Results:The UACR of DN rats were significantly higher than those in the normal group;kidney glomerulus mesangial cells and mesangial matrix increased markedly were increased.Shenning mixture I can reduce UACR;there was no difference with losartan;the effect of reducing UACR in the Shenning mixture I low dose group was superior to the Shenning mixture I high dose group.Compared with the Shenning mixture I,losartan can increase risk of kidney damage.The serum creatinine in the Shenning mixture I high dose group was obviously lower than that in the Shenning mixture I low dose group.The alleviating renal pathology of the Shenning mixture I is better than losartan.The proportion of Tregs in peripheral blood of DN rats were decreased;the Shenning mixture I and losartan can ameliorate the Tregs;there was no significant difference between groups.Conclusion:Shenning mixture I is a safer and more effective prescription.Shenning mixture I may alleviate DN renal injury by regulating Treg cells and inhibiting DN immune inflammatory reaction.
出处
《中医临床研究》
2017年第32期1-5,共5页
Clinical Journal Of Chinese Medicine
基金
中国中医科学院江苏分院院级课题-肾宁Ⅰ号合剂调节Th17/Treg平衡治疗糖尿病肾病研究(JSBY1314)