2017年辽宁省番茄灰霉病菌对腐霉利的抗药性现状及机制研究

Studies on Drug Resistance of Procymidone to Tomato Botrytis cinerea at Liaoning Province in 2017

从辽宁省8个地区的番茄种植区中采集、分离纯化得到番茄灰霉病(Botrytis cinerea)单孢菌株115株,采用菌丝生长速率法测定其对腐霉利的抗药性,并对抗药性分子机制进行研究。结果表明,辽宁省番茄灰霉病菌对腐霉利的抗药性呈现加重的趋势,8个地区番茄灰霉病菌对腐霉利的敏感性差异较大,EC50值分布在0.05~16.30 mg·L^(-1)之间,平均为3.99mg·L^(-1),抗性频率达到83.48%,以中抗菌株为主,但在阜新和盘锦地区检测到高抗菌株。对抗性菌株Bc OS1基因序列分析表明,所选抗性菌株在第369位和第373位的氨基酸同时发生突变。

A total of115single conidial isolates(Botrytis cinerea)were collected,isolated from8regions of Liaoning Province.Studies were carried out on determining Botrytis cinerea resistance to procymidone by mycelial growth rate method and molecular mechanism of the resistance.The findings indicated that the drug resistance of procymidone to tomato Botrytis cinerea had a tendency to accentuate in Liaoning Province,and there were big differences between sensitiveness of Botrytis cinerea to procymidone.The current work demonstrated that the EC50values of Botrytis cinerea to procymidone was between0.05-16.30mg·L-1,the average value was3.99mg·L-1and the resistance frequency was83.48%.Most of the resistant isolates showed middle resistance,and the high resistant isolates were found in Shenyang and Panjin areas.The analysis of resistant bacterial strain BcOS1gene sequencing indicated that the amino acid of the selected resistant bacterial strain silmotaneously mutated at the position of369and373.