盐酸四环素对大鼠骨髓间充质干细胞增殖的作用

Tetracycline hydrochloride promotes rat bone marrow mesenchymal stem cells proliferation in vitro

背景:研究表明,低浓度四环素可促进体外培养的人胚成骨细胞活性及增殖,增加且加强细胞合成Ⅰ型胶原、骨钙素等促进骨生成的物质。目的:观察盐酸四环素体外对大鼠骨髓间充质干细胞增殖的作用。方法:取第3代SD大鼠骨髓间充质干细胞,分2组干预,对照组加入含胎牛血清的完全培养基培养,实验组加入含盐酸四环素及胎牛血清的完全培养基培养。培养1-8 d,采用MTT法、细胞计数法检测两组细胞增殖活性。结果与结论:(1)细胞计数法:随着培养时间的延长,两组细胞数量逐渐增加,实验组培养4-8 d的细胞数量高于对照组(P<0.05);(2)MTT法:两组细胞数量均随培养时间的延长而增加,第1天为细胞潜伏期,3-6 d为对数生长期,第7天,对照组细胞进入平台期,实验组细胞继续增殖,实验组培养5-8 d的细胞增殖高于对照组(P<0.05);(3)结果表明:盐酸四环素可促进骨髓间充质干细胞的增殖。

BACKGROUND:Tetracycline hydrochloride is shown to promote viability and proliferation of human embryonic osteoblasts cultured in vitro,increase the production of type I collage and osteocalcin.OBJECTIVE:To study the proliferation activity of bone marrow mesenchymal stem cells(BMSCs)cultured by tetracycline hydrochloride in vitro.METHODS:Passage3BMSCs from Sprague-Dawley rats were cultured and divided into two groups:cells in control group were cultured in complete medium containing fetal bovine serum,and those in experimental group were cultured in complete medium containing tetracycline hydrochloride.After days1-8of culture,MTT and cell counting assay were used to detect cell viability and proliferation in the two groups.RESULTS AND CONCLUSION:(1)Cell counting assay:with time,the number of cells in both groups was increased,but the cell number in the experimental group was higher than that in the control group at days4-8of culture(P<0.05).(2)MTT assay:With time,the number of cells in both groups was increased:incubation period was at day1,logarithmic growth period was within days3-6,and plateau period began at day7.Then,the cells continued to proliferate,and the cell proliferation was higher in the experimental group than the control group at days5-8of culture(P<0.05).To conclude,tetracycline hydrochloride can significantly enhance BMSCs proliferation.