建立我国规模化无菌动物高效研究应用体系

Establishment of a highly effective large-scale germ-free animal efficiency research and application system in China

无菌动物与宏基因组技术是驱动人体健康微生物组研究的两大动力。没有无菌动物菌群(株)移植模型,就无法确立菌群与疾病的因果关系;没有无菌动物,就没有菌群与人体疾病研究飞速发展的今天与明天。无菌动物应用已形成有菌与无菌动物比较、菌群(株)移植、基因工程动物无菌化、无菌动物发育四种通用研究模式,研究模式的标准化将加大无菌动物研究应用规模及速度。本课题组经过十几年努力,已建成国内体量大、技术体系稳定、服务单位多、具有较大影响力的无菌动物平台,为国内生物医学、畜牧、食品微生物组科学问题解决提供了有力支撑。但是,目前我国无菌动物规模小、效率低、供用平台与应用条件分离,无菌动物基础理论与技术体系尚待发展,难以满足日益增长的应用需求。亟待建立规模化无菌动物高效研究应用体系,以适应我国微生物组研究高速发展。

Germ free animals and metagenomic technology are the two major wheels for the study of microbiome in human health.Without a transplantation model of germ free animals inoculated with microbiota(bacterial strains),it is impossible to establish the causal relationship between microbiota and diseases.Without germ free animals,there will be no rapid development of research on the relationship between microbiota and human diseases today and tomorrow.Application of germ free animals has formed four kinds of general research models,including comparison of conventional and germ free animals,bacteria(strain)transplantation with germ free animals,rederivation of germ free genetic engineering animals,and development of germ free animals.Standardization of research mode will increase the scale and speed of application of germ free animals.After ten years of efforts,Professor Wei Hong’s group has built the largest scale,the most stable technical system,most widely used platform of germ free animal in our country,which have provided a strong support in our country for scientific problem solving in the fields including animal husbandry,biomedicine,and food microbiology.At present,the germ free animals are in small scale,low in efficiency,and the platform is separated from the application conditions.The basic theory and technical system of germ free animals are still to be developed,and it is difficult to meet the growing demand for application.Establishment of an efficient large scale research and application system of germ free animals will meet the needs of rapid development of microbiome research in China.