下调STC2基因表达对前列腺癌细胞增殖及凋亡的影响研究

Effect of down regulation of STC2 gene expression on proliferation and apoptosis of prostate cancer cells

目的探讨抑制斯钙素-2(stanniocalcin-2,STC2)基因表达对前列腺癌细胞增殖及凋亡的影响。方法通过Lipofectamine TM2000脂质体介导法将siRNA阴性对照组和STC2-siRNA组转染人前列腺癌PC-3细胞,未转染的细胞作为空白对照组,48 h后收集细胞,Western bloting检测各组细胞中STC2的蛋白表达;CCK8及流式细胞仪分别检测细胞的增殖及凋亡情况;Western bloting检测增殖相关蛋白ki67、B细胞淋巴瘤/白血病-2(Bcl-2)家族Bcl-2和Bcl-2相关X蛋白(Bax)及PI3K/AKT信号通路磷脂酰肌醇3-激酶(PI3K)和磷酸化的丝氨酸苏氨酸激酶(pAKT)的蛋白表达。结果 STC2-siRNA转染PC-3细胞后STC2的蛋白表达显著低于空白对照组(P<0.05);阴性对照组细胞OD值及细胞凋亡率与空白对照组差异无统计学意义(P>0.05),而STC2-siRNA组细胞OD值显著低于空白对照组,凋亡率显著高于空白对照组(P<0.05);阴性对照组ki67、Bcl-2、Bax、PI3K和p-AKT蛋白表达与空白对照组差异无统计学意义(P>0.05),STC2-siRNA组ki67、Bcl-2、PI3K和p-AKT蛋白表达均显著低于空白对照组,Bax蛋白表达显著高于空白对照组(P<0.05)。结论 STC2基因表达的抑制可降低前列腺癌细胞的增殖,并诱导细胞凋亡,其机制与调节ki67、Bcl-2、Bax及PI3K/AKT信号通路表达有关。

ObjectiveTo investigate the effect of inhibiting of the STC2gene expression on proliferation and apoptosis of prostate cancer cells.MethodsHuman prostate cancer PC-3cells were transfected with siRNA negative control group and STC2-siRNA group by LipofectamineTM2000liposome mediated method,and untransfected cells served as blank control group,cells were collected after48h,the expression of STC2protein was detected by Western bloting;proliferation and apoptosis of cells were detected by CCK8and flow cytometry,respectively;Western bloting were used to detect ki67,Bcl-2,Bax,PI3K and p-AKT protein expression.ResultsSTC2protein expression after STC2-siRNA transfected PC-3cells was significantly lower than the blank control group(P<0.05).OD value and apoptosis rate between the blank control group and the negative control group had no significant difference(P>0.05),and OD value was significantly lower in STC2-siRNA group than the control group,the apoptosis rate was significantly higher than control group(P<0.05).ki67,Bcl-2,Bax,PI3K and p-AKT protein expression between negative control group and control group differences no statistical significance(P>0.05).The expression of ki67,Bcl-2,PI3K and p-AKT protein in STC2-siRNA griup were significantly lower than the control group,the expression of Bax protein was significantly higher than control group(P<0.05).ConclusionThe inhibition of STC2gene expression could reduce the proliferation of prostate cancer cells and induce apoptosis,and the mechanism is related to regulating the expression of ki67,Bcl-2,Bax and PI3K/AKT signaling pathways.