载药硼酸盐骨水泥的制备及其体外生物活性和抗菌性能的研究

The preparation, bioactivity and antibiotic property of drug loading borate bone cement

目的以硼酸盐生物活性玻璃和改性壳聚糖液相制备了新型的硼酸盐骨水泥,同时负载骨髓炎治疗药物硫酸庆大霉素,考察其体外抗菌性能,以探讨其治疗骨髓炎的可能性。方法以硼酸盐骨水泥为载体,制备了负载硫酸庆大霉素(GS)的骨水泥。探究负载GS对骨水泥的可注射性能、初凝时间的影响;将负载GS且预固化的硼酸盐骨水泥浸泡于磷酸盐缓冲溶液(PBS)中,考察其体外生物活性、生物降解性和药物释放;利用抑菌圈实验评估了负载GS的硼酸盐骨水泥的体外抗菌性能。结果制备的载药硼酸盐骨水泥能够被完全注射,初凝时间约6 min;在体外的磷酸盐缓冲溶液(PBS)中浸泡时,GS能够持续稳定的释放,药物释放长达26天;负载GS的硼酸盐骨水泥能够很好地抑制金黄色葡萄球菌(S.aureus)和大肠杆菌(E.coli)的生长。结论制备的负载GS的硼酸盐骨水泥具有优异的可注射性,合适的原位自固化时间,长期持续的药物释放和抗菌性能,可以用于骨髓炎治疗的进一步研究。

Objective The objective of this study was to develop a borate bone cement(BBC)that could serve as a carrier for delivering Gentamycin Sulfate(GS)and evaluate the capacity of antibiotic in vitro.Methods A local drug delivery system,using novel BBC as carrier for GS,was prepared.The effect of drug loading on the injectability,initial setting time of the injectable BBC was tested.The bioactivity,biodegradation and loading/release capacity of the BBC was evaluated by immersing the pre-set BBC samples in phosphate buffered saline,PBS.The in vitro antibiotic property was test by the inhibition zone of the E.coli and S.aureus cultured on the LB agar plates.Results The BBC loaded with GS can be almost completed injected by syringe,and had a proper initial setting time of about6min.When immersedin PBS,sustained release of GS occurred over26days,while the borate bioactive glass in BBC converted continuously to hydroxyapatite(HA).After loading with GS,the BBC can inhibit the growth of E.coli and S.aureus effectively.Conclusion The results suggest that,GS loaded BBC,had an excellent injectability,proper in-situ self-curing time,long and sustained drug release and the ability to inhibit bacterium growth.