中药有效组分调控p38MAPK改善糖尿病动脉粥样硬化兔炎症反应的机理研究

Effects of Traditional Chinese Medicine Composition on the Inflammatory Response of Atherosclerosis Rabbit Modle by Controlling p38MAPK

目的对中药组分(30%黄连生物碱、40%麦冬多糖、30%三七总皂苷)干预p38MAPK信号转导通路改善糖尿病动脉粥样硬化(diabetic atherosclerosis,DA)兔炎症反应进行研究。方法静脉推注四氧嘧啶并且配合腹主动脉内膜球囊损伤术诱导雄性纯种新西兰大白兔的DA模型,之后给予中药配伍组分高、中、低剂量组及辛伐他汀组干预。结果中药组分各组及辛伐他汀组对(p38mitogen-acti–vatedproteinkinase)p38MAPK表达有下调作用,中药组分高剂量组及辛伐他汀组可降低hs-CRP、IL-6、TNF-α、ET、ICAM-1、VCAM-1,与模型组比较差异有统计学意义(P<0.01);中剂量组可降低hs-CRP、IL-6、ET、VCAM-1,与模型组比较差异有统计学意义(P<0.01);低剂量组可降低hs-CRP,与模型组比较差异有统计学意义(P<0.05);中药组分高、中剂量组降低了FBG、GHB、GSP,与模型组比较差异有统计学意义(P<0.01);中药组分低剂量组降低了FBG,与模型组比较差异有统计学意义(P<0.05);与模型组比较,辛伐他汀组没有明显统计学差异(P>0.05)。结论中药组分可能通过干预p38MAPK信号转导通路,改善DA兔的炎症反应。

Objective The purpose of this study was to explore the effects of traditional Chinese medicine composition(TCMC)on inflammatory reaction of diabetic atherosclerotic rabbit modle by interventing p38 mitogen-acti – vatedproteinkinase(p38MAPK) signal pathway. Methods DA rabbits were established by alloxan intravenous injection with abdominal aorta intima balloon injury, then intervened by TCMC of high,medium,low dose or simvastatin respectively. Rusults The expression of p38MAPK in the combination of high,medium,low dose and simvastatin group was reduced,and the difference was statistically significant(P <0.01). The high dose group and simvastatin group had lower effects on hs-CRP, IL-6, TNF-α, ET, ICAM-1, VCAM-1 and the difference was statistically significant(P<0.01). The medium dose group can reduce hs-CRP,IL-6,TNF-α,ET,VCAM-1 and the comparison of the model group is statistically significant(P<0.01). The low-dose group decreased hs-CRP,and the difference was statistically significant(P<0.05). The high and medium-dose group of TCMC had reduced the FBG,GHB and GSP of the diabetic arteriosclerosis rabbits,significantly lower than the model group(P <0.01). The low-dose group of TCMC had a reduced effect on FBG, which was significantly lower than that in the model group(P <0.05). There was no statistically significant difference between simvastatin group and model groups(P >0.05). Conclution The traditional Chinese medicine composition may be able to inhibit inflammatory reaction of atherosclerotic rabbit modle by interventing p38 mitogen-acti –vatedproteinkinase(p38MAPK) signal pathway.