含臭参复方对实验性便秘的影响及作用机制研究

Influence of Compound Chinese Medicine Containing Codonopsis pilosula Produced in Yunnan Province on Purgation and Its Primary Action Mechanism

目的探讨臭参为君的复方对实验性便秘的影响及初步作用机制。方法通便实验中,复方地芬诺酯混悬液灌胃造成小鼠便秘模型,采用观察首便时间、便粒数、便重3项指标以观察含臭参复方的通便作用;并且采用小鼠炭末推进实验,来观察小肠推进运动。将小鼠随机分为对照组、模型组、不同给药剂量组(0.41g·kg^(-1)、0.82g·kg^(-1)、1.64g·kg^(-1)、3.28g·kg^(-1))。各组给药30min后,均以浓度为4%的墨汁进行灌胃,观察小鼠肠推进运动的变化。结果在通便实验中,首便时间、便粒数、便重3项指标中,模型组较空白组均有显著性降低(P<0.001),其中3.28g·kg^(-1)剂量组其首便时间与模型组比较显著缩短(P<0.05),便重与便粒数与模型组比较显著增加(P<0.01);小鼠炭末推进实验中,模型组的肠推进率较对照组显著降低(P<0.001),给药组的肠推进率显著高于模型组(P<0.05),给药5d的肠推进率高于给药1d和3d的效果,并呈现一定的量效关系或趋势。结论以云南产臭参为君的中药复方对实验性便秘具有改善作用,初步机理研究表明其可能与促进肠推进相关。

Objective To study the effects of compound Chinese medicine containing Codonopsis pilosula produced in Yunnanprovince(known as Choushen)on promoting digestion and purgation.Methods Mice were randomly divided into control group,modelgroup and different dosage groups(0.41g·kg-1,0.82g·kg-1,1.64g·kg-1,3.28g·kg-1).The time of the first stool,grain numbers of stool,stool weight in mice,the propulsion rate of intestine,after administration were observed.Results In the mice defecation test,the time of the first stool(227.75+38.82min)at the dosage of3.28g·kg-1was much shorter compared with the model group(269.38+44.41min)(P<0.05).The stool weight(0.537+0.203g)of3.28g·kg-1group was heavier versus the model group(0.242+0.178g)(P<0.01).In addition,the grain numbers of stool(22.63+6.14)of highest group also significantly increased when compared with model group(13.75+8.24)(P<0.01).In the mice digestion test,the intestinal propulsive rate of the treatment group was significantly higher than that of the model group(P<0.05),the intestinal propulsive rate of5-day administration was higher than that of1day or3days.Conclusion The present compound Chinese medicine containing C.pilosula possesses significant effects on purgation promotion and this effect might be associated with digestion promotion,suggesting its potential roles in the development of healthcare foods.