6个不同产地铁皮石斛功效成分的分析研究

Study on Functional Components of Dendrobium officinale from Different Culturing Areas

目的对比6个产地铁皮石斛功效成分的差异,以期为铁皮石斛进一步开发利用以及石斛行业的质量规范提供试验数据支撑。方法通过高效液相色谱法和紫外分光光度法分别测定不同产地3年生铁皮石斛的干燥茎中多糖、D-甘露醇、总黄酮、总皂苷、总生物碱、核苷类成分及甾族化合物等多种化学成分,并对6个产地进行Q-聚类分析。结果浙江省乐清市双峰乡所产石斛的多种功效成分相对较高,聚类结果显示昆明市官渡区所产石斛与文山广南县最为接近,德宏州盈江县石斛与其他产地相距最远。结论铁皮石斛功效成分众多,开发潜力极大,但各地区材料功效成分差异明显,而这些差异与地理位置关联不大,推测种质资源与人工繁育体系影响更大,说明急需统一的质量标准约束。

Objective By comparing the functional components of Dendrobium officinale from six areas to provide a theoretical basis for the further development of Dendrobium officinale and establishing a quality standard of industry.Methods Polysaccharide,D-mannitol,flavonoids,total saponins,total alkaloids,nucleosides and steroids in dry stems of Dendrobium officinale from different areas were detected by HPLC and UV experiments,and Q-cluster analysis was performed on6producing areas.Results Dendrobium officinale from Leqing of Zhejiang province has the relatively higher quality,the cluster diagram shows that the materials from Guandu district of Kunming and Guangnan county of Wenshan Prefecture has the nearest distance,and Yingjiang county of Dehong Prefecture is the farthest away from other producing areas.Conclusion Dendrobium officinale has many functional components and its potential is great,but the materials’differences are obvious,and these are not related to the geographical location.It is speculated that germplasm resources and artificial breeding system have great influence,which indicates the need for uniform quality standard.