糖尿病肾病患者血清CysC含量与氧化应激、肾间质纤维化的相关性研究

Correlation of serum CysC content with oxidative stress and renal interstitial fibrosis in patients with diabetic nephropathy

目的:研究糖尿病肾病患者血清CysC含量与氧化应激、肾间质纤维化的相关性。方法:选择在本院就诊的2型糖尿病患者作为研究对象,根据尿白蛋白/肌酐比(UACR)分为UACR<30mg/g·Cr的单纯2型糖尿病组(T2DM组)、UACR 30~300 mg/g·Cr的尿微量白蛋白组(MA组)、UACR>300mg/g·Cr的尿大量白蛋白组(LA组)。检测血清中CysC、氧化应激指标的含量以及尿液中肾间质纤维化的含量。结果:MA组、LA组血清中CysC、NOX4、MDA的含量以及尿液中Col-IV、FN、TGF-β、CTGF的含量显著高于T2DM组,HO-1、SOD、GSH-Px的含量显著低于T2DM组;LA组患者血清中CysC、NOX4、MDA的含量以及尿液中Col-IV、FN、TGF-β、CTGF的含量显著高于MA组,HO-1、SOD、GSH-Px的含量显著低于MA组;CysC升高的糖尿病肾病患者血清中NOX4、MDA的含量以及尿液中Col-IV、FN、TGF-β、CTGF的含量显著高于CysC正常的糖尿病肾病患者,HO-1、SOD、GSH-Px的含量显著低于CysC正常的糖尿病肾病患者。结论:糖尿病肾病患者血清CysC含量的升高与氧化应激反应、肾间质纤维化过程的激活密切相关。

Objective:To study the correlation of serum CysC content with oxidative stress and renal interstitial fibrosis in patients with diabetic nephropathy.Methods:Patients with type2diabetes mellitus who were treated in Yangxin People's Hospital between May2013and February2017were selected as the research subjects,and according to the urinary albumin/creatinine ratio(UACR),they were divided into the simple type2diabetes mellitus group(T2DM group)with UACR<30mg/g·Cr,microalbuminuria group(MA group)with UACR30~300mg/g·Cr and macroalbuminuria group(LA group)UACR>300mg/g·Cr.The contents of CysC and oxidative stress indicators in serum as well as the contents of renal interstitial fibrosis indicators in urine were detected.Results:Serum CysC,NOX4and MDA contents as well as urine Col-IV,FN,TGF-βand CTGF contents in MA group and LA group were significantly higher than those in T2DM group while HO-1,SOD and GSH-Px contents were significantly lower than those in T2DM group;serum CysC,NOX4and MDA contents as well as urine Col-IV,FN,TGF-βand CTGF contents in LA group were significantly higher than those in MA group while HO-1,SOD and GSH-Px contents were significantly lower than those in MA group;serum NOX4and MDA contents as well as urine Col-IV,FN,TGF-βand CTGF contents in diabetic nephropathy patients with high CysC were significantly higher than those in diabetic nephropathy patients with normal CysC while HO-1,SOD and GSH-Px contents were significantly lower than those in diabetic nephropathy patients with normal CysC.Conclusion:The increase of serum CysC content in patients with diabetic nephropathy is closely related to the activation of oxidative stress response and renal interstitial fibrosis.