滇重楼种子内源抑制物质的活性研究

Activity of Endogenous Inhibitor Substances in Seeds of Paris polyphylla var. yunnanensis

以滇重楼种皮和胚乳为材料,用甲醇浸提得到种皮浸提液和胚乳浸提液,配制成浓度梯度为0.02、0.04、0.06、0.08g/mL的处理液,以蒸馏水为对照,研究不同浓度种皮和胚乳浸提液对受体植物白菜(油脂类)、小麦(淀粉类)、绿豆(蛋白类)种子发芽率、幼苗苗高和根长、抗氧化酶活性的影响,探讨滇重楼种子内源抑制物质的特性,以揭示滇重楼种子休眠的生理机制。结果表明:(1)滇重楼种皮和胚乳中均含有抑制种子萌发与幼苗生长的物质,且抑制作用大小表现为胚乳大于种皮。(2)3种受体植物幼苗对滇重楼种皮、胚乳甲醇浸提液的敏感性大小为白菜(油脂类)显著高于绿豆(蛋白类)和小麦(淀粉类)。(3)滇重楼种皮、胚乳甲醇浸提液对种子萌发及幼苗生长各项指标的抑制作用强度依次为:发芽率>幼苗根长>幼苗苗高>鲜重。(4)滇重楼种皮、胚乳中的抑制物质均能够使3种受体植物幼苗产生生理响应,启动抗氧化酶体系,其中:随着滇重楼种皮甲醇浸提液浓度的升高,受体幼苗SOD与POD活性先升后降,CAT活性持续下降;胚乳甲醇浸提液处理后,受体幼苗SOD和POD活性较对照均显著增强,而CAT活性显著下降。研究认为,滇重楼种皮及胚乳甲醇浸提液均能够抑制受体植物种子萌发及幼苗生长,影响幼苗抗氧化酶活性,但滇重楼种皮与胚乳中所含抑制物在活性上存在差异。

To study the reasons for dormancy and characteristics of endogenous inhibitory substances of Paris polyphylla var.yunnanensis,seed germination,seedlings growth and antioxidant enzymes of three kinds of crops in different concentrations of we studied the methanol extract from P.polyphylla var.yunnanensis seed coat and endosperm in order to investigate the relationship between the restraining substraces in seed coat and endosperm in dormancy of P.polyphylla var.yunnanensis Hope to provide scientific reference for breaking P.polyphylla var.yunnanensis seed dormancy.The results showed that:(1)there are endogenous inhibitory both in seed coat and endosperm,and the inhibitory action from endosperm is stronger than that of seed coat.(2)Three receptor plant seedlings show different sensibility to the endogenous inhibitory of seed coat and endosperm,Brassica campestris L.ssp.pekinensis(Oil)seeds are significantly more sensitive than Triticum aestivum(Starch)seeds and Vigna radiata(Protein)seeds.(3)Inhibition by P.polyphylla var.yunnanensis seed coat and endosperm methanol extraction,the strength of growth indexes about seed germination and seedling are rating as:germination rate>seedling root length>seedling height>fresh weight.(4)The inhibitory substances in the seed coat and endosperm cause the physiological response of the three receptor plant seedlings,and start the antioxidant enzyme system.With the increase of seed coat methanol extract concentration,the activities of superoxide dismutase(SOD)and peroxidase(Pod)increased first and then decreased,while catalase(CAT)activity continued to decline.After treatment with methanol extract from endosperm,the activities of SOD and POD were significantly increased compared with control group,and CAT activity decreased significantly.The study found that there are chemical components both in seed coat and endosperm of P.polyphylla var.yunnanensis,These chemicals inhibited the seed germination and seedling growth of recipient plants,affected the activities of antioxidant enzymes in seedlings.However,there were differences in the activity of the inhibitors contained in seed coat and endosperm.