健脾益智胶囊对大脑中动脉栓塞大鼠脑梗死体积和ATP酶活性的影响

Effect of Jianpi Yizhi Capsule on Cerebral Infarction Volume and ATP Enzyme Activity in Rats with Middle Cerebral Artery Occlusion

[目的]探讨健脾益智胶囊对大脑中动脉栓塞(MCAO)大鼠脑梗死体积和ATP酶活性的影响。[方法]选取135只SD大鼠随机分为正常组、模型组、中药组,采用线栓法建立MCAO大鼠模型,中药组予健脾益智胶囊干预,各组于术后6 h、12 h、1 d、3 d、7 d,采用TTC染色法计算脑梗死体积,采用定磷法测定大鼠脑组织中Ca2+-ATP酶和Na+-K+-ATP酶的活性水平。[结果]中药组梗死体积与模型组比较显著缩小,模型组术后1 d的梗死体积最大(P<0.05);术后6 h、12 h、1 d、3 d,与正常组相比,模型组术后Na+-K+-ATP酶活性显著降低(P<0.05);术后6 h、12 h、7 d,与模型组相比,中药组Na+-K+-ATP酶活性显著增强(P<0.05)。术后6 h、12 h、3 d与正常组相比,模型组Ca2+-ATP酶活性显著降低(P<0.05);术后6 h、3 d、7 d,与模型组相比,中药组Ca2+-ATP酶活性显著增强(P<0.05)。[结论]健脾益智胶囊可缩小大鼠脑梗死体积,可能与增加ATP酶活性有关。

[Objective]To investigate the effect of Jianpi Yizhi capsule on cerebral infarction volume and ATP enzyme activity in rats with middle cerebral artery occlusion(MCAO).[Methods]One hundred and thirty-five SD rats were randomly divided into normal group,model group,Chinese medicine group.The MCAO rat model was established by thred occlusion.The Chinese Medicine group was intervened with Jianpi Yizhi capsule.The volume of cerebral infarction was calculated by TTC staining and the activity of Ca2+-ATPase and Na+-K+-ATPase in brain tissue of rats for each group were measured by fixed phosphorus method at6h,12h,1d,3d and7d after operation.[Results]The infarct volume of Jianpi Yizhi group was significantly reduced compared with that of model group.The model group had the largest infarction volume on the first postoperative day(P<0.05).The activity of Na+-K+-ATPase in the model group decreased significantly at6h,12h,1d and3d after operation compared with that in the normal group(P<0.05).The activity of Na+-K+-ATPase in the Chinese medicine group increased significantly at6h,12h and7d after operation compared with that in the model group(P<0.05).The activity of Ca2+-ATPase in the model group was significantly lower at6h,12h and3d after operation compared with that in the normal group(P<0.05).The activity of Ca2+-ATPase in Chinese medicine group increased significantly at6h,3d and7d after operation compared with that in the model group(P<0.05).[Conclusion]Jianpi Yizhi capsule can reduce the volume of cerebral infarction in rats,which may be related to the increase of ATPase activity.